A Preliminary Study on the Changes of Neurotransmitters in Neural Pathways Related to Tinnitus Induced by Salicylate in Rats
10.3969/j.issn.1006-7299.2025.01.014
- VernacularTitle:水杨酸盐致大鼠耳鸣相关神经通路递质变化的初步研究
- Author:
Bin YI
1
;
Qingping MA
1
;
Xueling WANG
1
;
Huihui LIU
1
;
Jiayi GU
1
;
Tingting DU
1
;
Wenxiao WANG
1
;
Runjie SHI
1
;
Zhiwu HUANG
1
Author Information
1. 上海交通大学医学院附属第九人民医院耳鼻咽喉-头颈外科(上海 200011);上海交通大学医学院耳科学研究所;上海市耳鼻疾病转化医学重点实验室
- Publication Type:Journal Article
- Keywords:
Tinnitus;
Salicylate;
Auditory center;
Neurotransmitter;
Neuroplasticity
- From:
Journal of Audiology and Speech Pathology
2025;33(1):66-72
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the dynamic changes in the secretion of neurotransmitters glutamic acid(Glu)and γ-aminobutyric acid(GABA)in the central auditory brain area,in order to explore the effects of sodium salicy-late on different locations of the auditory pathway.Methods A total of 126 SD rats were injected intraperitoneally with salicylate,and were divided into 10 groups including injection groups for 1,2,4,8,and 24 hours,chronic in-jection groups for 3,7,and 14 days,and chronic recovery groups for 21 and 28 days with 6 rats in each group,as well as their corresponding blank control groups.Rats in each group were anesthetized and materials were collected for further use.High-performance liquid chromatography(HPLC)was performed to detect and compare the dynam-ic changes in the levels of Glu and GABA in the auditory cortex,inferior colliculus,cochlear nucleus,and hippocam-pus of the auditory center of rats in each group at different time points.Results Compared with the control group,within 24 hours of acute injection of salicylate,the Glu content in the auditory cortex reached the peak in 1 hour,and the hippocampus reached the peak at the 4th hour after injection,and then decreased slowly.The GABA con-tent in the four brain regions showed a slow upward trend in the chronic injection period,reached the peak on the 7th day,decreased and approached normal level on the 14th day,and basically returned to the normal level in the re-covery period.Conclusion These findings indicate that salicylate has a certain short-term excitatory and stimulating effect on the central auditory system.Under the mechanism of central plasticity,after long-term injection of salicy-late,the release of neurotransmitters reaches a new excitation/inhibition balance in the central area.Glu and GABA may each play a different role that may ultimately lead to the development of tinnitus.
