Mechanism of Congrong Shujing granules in treatment of Parkinson's disease based on network pharmacology,molecular docking and parallel reaction monitoring technology
- VernacularTitle:基于网络药理学、分子对接与PRM靶向蛋白验证探讨苁蓉舒痉颗粒治疗帕金森病的作用机制
- Author:
Hai-xin LIU
1
;
Hui-xin NI
;
Mei ZHOU
;
Zi-li FAN
;
Zheng-tao GAO
;
Fang-zhen WU
;
Yao LIN
;
Qian XU
;
Jing CAI
Author Information
- Publication Type:Journal Article
- Keywords: Parkinson's disease; Congrong Shujing granule(CSGs); network pharmacology; molecular docking; PRM; ASK1-JNK pathway
- From: Chinese Pharmacological Bulletin 2025;41(2):365-372
- CountryChina
- Language:Chinese
- Abstract: Aim To explore the mechanism of Con-grong Shujing granule(CSGs)in the treatment of Par-kinson's disease(PD)by network pharmacology,mo-lecular docking and parallel reaction monitoring(PRM)technology.Methods The active components of CSGs and the target genes of Parkinson's disease were obtained through the database.The intersection targets of drugs and diseases were selected to construct the"drug-active ingredient-target"and protein interac-tion network.The intersection target genes were impor-ted into David database for GO and KEGG enrichment analysis,and the main components were docked with key targets.27 SD rats were randomly divided into the normal group(n=9),model group(n=9)and treat-ment group(n=9).On day 1,7 and 14 of treatment,PRM analysis was used to detect the changes in the specific peptides of key target proteins in the substantia nigra of rats.Results The main components of CSGs wereTanshialdehyde,Baicalein,Quercetin and Kaempferol.The most important targets for the treat-ment of PD were TP53,AKT1,EGFR,HSP90 AA1 and STAT3.KEGG analysis mainly enriched MAPK,PI3K-Akt and neurotrophic factor signaling pathway.The molecular docking between core components and core targets showed that the binding of drugs and targets had good activity.PRM analysis of key proteins found that the target peptide expression levels of ASK1,JNK1 and JNK3 were different among groups(P＜0.05).Con-clusion CSGs can alleviate ERS,inhibit apoptosis and play a neural protective role through the ASK1-JNK pathway.
