Colonization and transmission of carbapenem-resistant Klebsiella pneu-moniae in intensive care unit
10.12138/j.issn.1671-9638.20255449
- VernacularTitle:重症监护病房耐碳青霉烯类肺炎克雷伯菌定植与传播研究
- Author:
Xiang CHEN
1
;
Xiaodong GAO
;
Chunmei ZHOU
;
Shenglei HUANG
;
Wenyan PAN
;
Xiao LIU
;
Jinghua MEI
;
Qingfeng SHI
Author Information
1. 复旦大学附属中山医院感染管理科,上海 200032;复旦大学医院感染与控制研究所,上海 200032
- Publication Type:Journal Article
- Keywords:
intensive care unit;
carbapenem-resistant Klebsiella pneumoniae;
whole-genome sequencing;
trans-mission
- From:
Chinese Journal of Infection Control
2025;24(1):77-84
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore and analyze the characteristics and transmission routes of carbapenem-resistant Klebsiella pneumoniae(CRKP)strains in intensive care unit(ICU).Methods From January to October 2023,17 clinical infection isolates(clinical infection group),5 active screening isolates(active screening group),and 7 envi-ronmental isolates(environmental group)of CRKP in the liver surgery ICU of a hospital were selected and analyzed by whole-genome sequencing.The differences in resistance genes,virulence genes,and sequence typing(ST)were compared,and transmission routes were analyzed based on the phylogenetic tree.Results 29 strains of CRKP car-ried 4-18 resistance genes and 52-98 virulence genes,respectively.There were no statistically significant diffe-rences in genotype distribution of resistance genes,the number of virulence genes,and gene types among three groups of CRKP(all P＞0.05).ST showed that 29 CRKP strains mainly consisted of two categories:ST11 and ST15.Based on the phylogenetic tree constructed from the core genome,there were 7 highly homologous groups of CRKP,among which 4 groups had clear epidemiological associations.Conclusion CRKP in ICU carries more re-sistance and virulence genes,and some strains are highly homologous in ST and phylogenetic tree,which may lead to cross transmission.In the future,prevention and control measures should be strengthened to reduce the trans-mission of CRKP.
