Antimicrobial activity of garvicin LG6 against Staphylococcus aureus of different hemolytic phenotypes
10.12138/j.issn.1671-9638.20256333
- VernacularTitle:Garvicin LG6对不同溶血表型金黄色葡萄球菌的抗菌活性
- Author:
Wei TANG
1
;
Shengnan WENG
;
Yawu WANG
;
Jie YAO
;
Xin LI
;
Qiang ZHOU
;
Yuanhong XU
Author Information
1. 安徽医科大学第一附属医院检验科,安徽 合肥 230022;安徽医科大学第二附属医院检验科,安徽 合肥 230601
- Publication Type:Journal Article
- Keywords:
Lactococcus garvieae;
bacteriocin;
Staphylococcus aureus;
hemolytic phenotype;
antimicrobial activity
- From:
Chinese Journal of Infection Control
2025;24(1):23-29
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the antimicrobial activity of the bacteriocin(tentatively named garvicin LG6)se-creted by Lactococcus garvieae(L.garvieae)SHAMU-LG6 against Staphylococcus aureus(S.aureus)of different hemolytic phenotypes.Methods S.aureus isolated from clinical patients in a hospital of Anhui from 2021 to 2023 were collected.The hemolytic phenotypes of S.aureus were detected by three-point inoculation method.S.aureus of different hemolytic phenotypes were further categorized into methicillin-sensitive S.aureus(MSSA)and methi-cillin-resistant S.aureus(MRSA)according to antimicrobial susceptibility testing results.The antagonistic activity of L.garvieae SHAMU-LG6 against S.aureus of different hemolytic phenotypes was assayed by Oxford cup me-thod.The whole-genome sequencing of L.garvieae SHAMU-LG6 was performed.Biosynthetic gene cluster of gar-vicin LG6 was searched by online databases antiSMASH 7.0 and BAGEL4.Through macroporous resin adsorption,ethanol gradient elution,rotary evaporation,and dried material reconstitution,antimicrobial activity of garvicin LG6 crude extract against S.aureus was detected by the inhibitory testing of Oxford cup method.Results L.garvieae SHAMU-LG6 could significantly antagonize MSSA and MRSA of different hemolytic phenotypes.Biosynthetic gene cluster of garvicin LG6 was present on the chromosomal genome of L.garvieae SHAMU-LG6.The antimicrobial activity of garvicin LG6 secreted by a single colony or 6 mL fermentation fluid of L.garvieae SHAMU-LG6 were at least equal to that of antibiotic disc of 30 pg cefoxitin.Conclusion Garvicin LG6 can efficiently kill MSSA and MR-SA of different hemolytic phenotypes,and has the potential to be developed into a novel antimicrobial agent,which has great prospects for clinical application.
