Application of centrifugal microfluidic chips based on loop-mediated isothermal amplification technology for rapid detection of foodborne pathogens
10.3760/cma.j.cn341190-20240507-00521
- VernacularTitle:基于环介导等温扩增技术的离心式微流控芯片在食源性致病菌快速检测中的应用
- Author:
Meng YANG
1
;
Wei LIU
1
;
Tiao BAI
1
;
Lingling WANG
1
Author Information
1. 延安市疾病预防控制中心检验科，延安 716000
- Publication Type:Journal Article
- Keywords:
Foodborne diseases;
Bacteria;
Culture techniques;
ROC curve;
Microchip analytical procedures
- From:
Chinese Journal of Primary Medicine and Pharmacy
2025;32(2):250-255
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the application value of centrifugal microfluidic chips based on loop-mediated isothermal amplification (LAMP) technology for rapid detection of foodborne pathogens.Methods:A retrospective analysis was conducted on 128 test samples collected by the Yan'an Center for Disease Control and Prevention from January 2022 to January 2024. All samples underwent bacterial culture and testing using centrifugal microfluidic chips based on LAMP technology. The detection time, spiking concentration, and accuracy of two detection methods were compared. Additionally, the diagnostic efficacy of both methods was analyzed using receiver operating characteristic (ROC) curves.Results:Bacterial cultures were mostly detected within 16-48 hours, with a detection limit concentrated at 10 000 CFU/25 g. In contrast, the LAMP-based centrifugal microfluidic chip technology typically yielded results within 5-16 hours, with a detection limit ranging from 1 000 to 10 000 CFU/25 g. The differences were statistically significant ( t = 14.86, 573.04, both P < 0.001). When compared with subsequent confirmation results, the positive rate for bacterial culture was 75.00% (24/32), with a diagnostic accuracy of 80.47% (103/128), showing a low consistency (Kappa = 0.51, P < 0.001). Meanwhile, the LAMP-based centrifugal microfluidic chip technology had a positive detection rate of 93.75% (30/32) and a diagnostic accuracy of 96.87% (124/128), also exhibiting low consistency (Kappa = 0.92, P < 0.001). In paired comparisons, the difference between the LAMP-based centrifugal microfluidic chips and bacterial culture was statistically significant ( χ2 = 53.14, P < 0.001). ROC curve analysis indicated that the area under the curve for bacterial culture in diagnosing foodborne pathogens was 0.77, while the AUC for LAMP-based centrifugal microfluidic chip technology was 0.95. Conclusions:When detecting foodborne pathogens, the use of LAMP-based centrifugal microfluidic chip technology allows for the rapid identification of pathogens in a short time period, offering advantages such as high efficiency, speed, and accuracy.
