Stat1 inhibits Foxp3 expression and Treg production
10.3969/j.issn.1000-484X.2025.02.003
- VernacularTitle:Stat1抑制Foxp3的表达和Treg产生
- Author:
Shuai GUO
1
;
Ge ZHANG
;
Hao CHEN
;
Anqi QIN
;
Wenting LI
;
Aihong ZHANG
;
Aihua ZHENG
;
Feng TIAN
;
Quanhui ZHENG
Author Information
1. 华北理工大学基础医学院河北省慢性疾病基础医学重点实验室,唐山 063210
- Publication Type:Journal Article
- Keywords:
Stat1;
Foxp3;
Treg;
Human breast cancer cell;
MCF-7
- From:
Chinese Journal of Immunology
2025;41(2):271-275
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the influence of Stat1 on Foxp3 expression and production of Treg.Methods:C57BL/6 mice were used and separated into normal control group and Stat1 specific inhibitor Fludarabine(Flud)treatment group.Ratio of CD4+Foxp3+Treg and expression of Foxp3 in spleen,lymph nodes and peripheral blood of mice in each group were detected by flow cy-tometry.Human Stat1 overexpression plasmid was constructed and transfected into human breast cancer MCF-7 cells,and expression changes of Foxp3 was detected by RT-qPCR and Western blot.Results:Compared with mice in normal control group,proportion of Treg and expression of Foxp3 in lymph nodes and peripheral blood of mice in Flud treatment group were increased,while Stat1 overex-pression resulted in decreased Foxp3 mRNA and protein expression in MCF-7 cells.Conclusion:Stat1 inhibits expression of Foxp3 and production of Tregs.
