Effect of circVMA21 targeting miR-497-5p/MUC1 axis on expressions of inflammatory factors and apoptosis of lung epithelial cells induced by LPS
10.3969/j.issn.1000-484X.2025.02.014
- VernacularTitle:circVMA21靶向miR-497-5p/MUC1轴对LPS诱导的肺上皮细胞炎症因子表达和细胞凋亡的影响
- Author:
Guoyuan LIU
1
;
Yuhua LIU
;
Xufang YANG
Author Information
1. 忻州市第二人民医院儿科,忻州 034100
- Publication Type:Journal Article
- Keywords:
Lung epithelial cells;
circVMA21;
miR-497-5p;
Mucin1;
Inflammation;
Apoptosis
- From:
Chinese Journal of Immunology
2025;41(2):342-350
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate whether circular RNA VMA21(circVMA21)targeting miR-497-5p/mucin1(MUC1)affecting the expressions of inflammatory factors and apoptosis in lung epithelial cells induced by LPS.Methods:A549 lung epithelial cells were divided into Con group(control),LPS group(LPS injury),LPS+pcDNA group,LPS+pcDNA-circVMA21 group,LPS+anti-miR-NC group,LPS+anti-miR-497-5p group,LPS+pcDNA-circVMA21+miR-NC group,LPS+pcDNA-circVMA21+miR-497-5p group.Expressions of circVMA21 and miR-497-5p were measured by qRT-PCR,expressions of inflammatory factors IL-6,IL-1β and TNF-α were determined by ELISA,flow cytometry was used to monitor cell apoptosis,and Western blot was employed to assay expres-sions of MUC1,TLR4/NF-κB pathway and apoptosis-related activation-cleaved-caspase3 and cleaved-caspase9 protein.The targeting relationships between circVMA21 and miR-497-5p,miR-497-5p and MUC1 were determined by dual luciferase reporter assay.Results:Compared with Con group,expressions of circVMA21 and MUC1 in lung epithelial cells of LPS group were decreased,while expression levels of miR-497-5p,IL-6,IL-1β and TNF-α,apoptosis rate,expressions of apoptotic proteins cleaved-caspase3 and cleaved-caspase9,and TLR4,p-NF-κB p65/NF-κB p65 were increased(P<0.05).Compared with LPS+pcDNA group,expressions of circVMA21 and MUC1 in lung epithelial cells of LPS+pcDNA-circVMA21 group were increased,while expression levels of IL-6,IL-1β and TNF-α,apoptosis rate,expressions of cleaved-caspase3 and cleaved-caspase9,and TLR4,p-NF-κB p65/NF-κB p65 were decreased(P<0.05).circVMA21 was targeted regulating the expression of miR-497-5p.Compared with LPS+anti-miR-NC group,ex-pressions of MUC1 in lung epithelial cells of LPS+anti-miR-497-5p group was increased,while expression levels of IL-6,IL-1β,TNF-α,apoptosis rate,protein cleaved-caspase3 and cleaved-caspase9,and TLR4,p-NF-κB p65/NF-κB p65 were decreased(P<0.05).miR-497-5p was targeted regulating the expression of MUC1.Compared with LPS+pcDNA-circVMA21+miR-NC group,expression of MUC1 in lung epithelial cells of LPS+pcDNA-circVMA21+miR-497-5p group was decreased,while expression levels of IL-6,IL-1β,TNF-α,apoptosis rate,expressions of cleaved-caspase3 and cleaved-caspase9,and TLR4,p-NF-κB p65/NF-κB p65 were increased(P<0.05).Conclusion:circVMA21 may promote expression of MUC1 and inhibit activation of TLR-4/NF-κB pathway by down-regu-lating miR-497-5p,thereby reducing expressions of inflammatory factors and apoptosis of lung epithelial cells induced by LPS.
