Optimization of extraction process for Jiangzhi Ligan Decoction and its effects on improving lipid deposition in HepG2 cells
10.3969/j.issn.1001-1528.2025.01.006
- VernacularTitle:降脂理肝汤提取工艺优化及其对HepG2细胞脂质蓄积的改善作用
- Author:
Jing JI
1
;
Yan-hua SUN
1
;
Fu-qiang HOU
1
;
Hao JIANG
1
;
Shan HE
1
Author Information
1. 山东中医药大学,山东济南 250355
- Publication Type:Journal Article
- Keywords:
Jiangzhi Ligan Decoction;
extraction process;
Box-Behnken response surface method;
HepG2 cells;
lipid deposition;
non-alcoholic fatty liver disease
- From:
Chinese Traditional Patent Medicine
2025;47(1):36-42
- CountryChina
- Language:Chinese
-
Abstract:
AIM To optimize the extraction process for Jiangzhi Ligan Decoction,and to investigate its effects on improving lipid deposition in HepG2 cells.METHODS With extraction time,extraction frequency and solvent consumption as influencing factors,comprehensive score for tanshensu,lotine,catechin contents and extract yield as an evaluation index,the extraction process was optimized by Box-Behnken response surface method on the basis of single factor test.Palmitic acid-induced HepG2 cells were adopted in the establishment of non-alcoholic fatty liver disease model,after which the effect of drug-containing serum on TC and TG levels was investigated.RESULTS The optimal conditions were determined to be 90 min for extraction time,three times for extraction frequency,and 11 times for solvent consumption.Compared with the control group,the model group demonstrated increased TG,TC levels(P＜0.01);compared with the model group,the Jiangzhi Ligan Decoction high-dose group displayed decreased TG,TC levels(P＜0.05,P＜0.01),especially for those after optimization(P＜0.05,P＜0.01).CONCLUSION Jiangzhi Ligan Decoction can alleviate lipid deposition in HepG2 cells induced by palmitic acid,whose efficacy after extraction process optimization is better than that reported in literature.
