Explore the Mechanism of Gegen Qinlian Decoction in Ameliorating Nonalcoholic Fatty Liver Disease Through Effect HepG2 Cells Based on Transcriptomics
10.3870/j.issn.1004-0781.2025.10.002
- VernacularTitle:基于转录组学探讨葛根芩连汤通过干预HepG2细胞改善非酒精性脂肪性肝病的作用机制
- Author:
Ailan WU
1
;
Yingqian CAO
;
Peiyao XIE
;
Zheng CAO
;
Shuhong PENG
;
Ziwen CHENG
;
Lan CAO
;
Changhua ZHANG
;
Fang LIANG
Author Information
1. 江西中医药大学药学院,南昌 330004
- Publication Type:Journal Article
- Keywords:
Gegen qinlian decoction;
Transcriptomics;
Nonalcoholic fatty liver disease;
HepG2 cells
- From:
Herald of Medicine
2025;44(10):1531-1540
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the potential mechanism of Gegen qinlian decoction(GGQLD)containing serum in ameliorating nonalcoholic fatty liver disease(NAFLD)in human hepatocellular carcinoma HepG2 cells based on transcriptomics.Methods An in vitro model of NAFLD was constructed by free fatty acid(FFA)-induced fat accumulation in HepG2 cells,and cells were treated with different proportions of GGQLD and pioglitazone-containing serum.The lipid deposition in each group was detected by oil red O staining,and the lipid content in each group was evaluated by triglyceride level.Transcriptome technology was used to detect the differentially expressed genes between the intervention groups,and GO annotation analysis,KEGG enrichment analysis and protein interaction(PPI)network analysis were performed to verify the differentially expressed genes by RT-PCR.Results Compared with normal control group,the number of red lipid droplets in the model control group increased,and the triglyceride content increased significantly(P＜0.01).Compared with model control group,the content of red lipid droplets in the GGQLD medium dose group showed a decreasing trend,and the intracellular triglyceride content decreased significantly(P＜0.05).A total of 608 differentially expressed genes were identified by transcriptome analysis,of which 163 differentially expressed genes were up-regulated and 445 differentially expressed genes were down-regulated.GO enrichment analysis showed that the differentially expressed genes were mainly involved in the regulation of MAP kinase phosphatase activity.KEGG analysis showed that the differentially expressed genes were mainly involved in MAPK signaling pathway.RT-PCR results showed that GGQLD up-regulated the expression level of MAP2K6 mRNA and down-regulated the expression levels of FOSL1,CTSL,DUSP5,DUSP1,JUN,HSPA6,IL1A,IL11 and RELB mRNA,which may be mainly involved in MAPK signaling pathway.Conclusion GGQLD has the effect of improving NAFLD,which may be related to MAPK signaling pathway.
