Study on the Influence of Different Honey Preparation Methods on Dissolution of Willow Leaf Baiqian in vitro
- VernacularTitle:柳叶白前不同蜜制方法对体外溶出度的影响研究
- Author:
Chunlong XUE
1
;
Yating WANG
1
;
Xiya ZHANG
1
;
Zhengyan DONG
1
;
Yinan WANG
1
;
Yuhang GUO
1
;
Xiujun DUAN
1
Author Information
- Publication Type:Journal Article
- Keywords: Cynanchum stauntonii(Decne)Schltr.ex Lévl.; Daucosterol; β-sitosterol; Glucose; Fructose
- From: World Science and Technology-Modernization of Traditional Chinese Medicine 2024;26(9):2415-2424
- CountryChina
- Language:Chinese
- Abstract: Objective To evaluate the effect of different honey processing methods on the dissolution of Cynanchum salicifolia in vitro.Methods High performance liquid chromatography ① Carotene and β-Determination method of sitosterol content:chromatographic column:Dia monosil C18(250 mm×4.6 mm,5 μm).Mobile phase:methanol:0.05％phosphoric acid water=99∶1.Isocratic elution;Flow rate 1.0 mL·min-1;Detection wavelength 209 nm;Injection volume 10 μL.The column temperature is 30℃.②Determination method of glucose and fructose:the chromatographic column is Inspire NH2 sugar analysiscolumn(250 mm×4.6 mm,5 μm).The mobile phase was acetonitrile water(77∶23).The flow rate is 0.8 mL·min-1;The column temperature is 35℃;Injection volume 10 μL;Evaporative light scattering detector(ELSD):the drift tube temperature is 100℃,and the carrier gas flow rate is 2.8 L·min-1.Results ①The results showed that carotene and β-the determination of sitosterol showed a good linear relationship in the range of mass concentration(r＞0.9999).The average recoveries were 99.71％and 98.83％.The RSD of precision,stability and repeatability were all less than 2.0％.②The established method of glucose fructose showed a good linear relationship in the range of 0.05-0.50 mg·mL-1(r=0.9991).Conclusion The content determination method and in vitro dissolution test methodology of the study meet the requirements,which provides a scientific basis for the establishment of quality standards and modern research of honey roasted Paeonia salicifolia.
