Proteomics study of lethal arrhythmias in rats
10.13618/j.issn.1001-5728.2025.04.011
- VernacularTitle:致死性心律失常大鼠的蛋白质组学研究
- Author:
Yuebing HUANG
1
;
Hai QIU
1
;
Wen CHEN
1
;
Zilin MENG
1
;
Yu CAI
1
;
Xia YUE
1
;
Dongfang QIAO
1
Author Information
1. 南方医科大学法医学院,广东 广州 510515
- Publication Type:Journal Article
- Keywords:
Forensic pathology;
Cause of death;
Sudden cardiac death;
Proteomics;
Arrhythmia
- From:
Chinese Journal of Forensic Medicine
2025;40(4):444-449
- CountryChina
- Language:Chinese
-
Abstract:
Objective To identify key proteins associated with sudden cardiac death(SCD)caused by lethal arrhythmia and to explore their potential molecular mechanisms through integrated proteomic analysis,data mining,and bioinformatics.Methods A lethal arrhythmia model was established in 8-week-old male Sprague-Dawley rats,which were divided into an arrhythmia group and a control group.Proteomic techniques were applied to identify and quantify proteins in left ventricular myocardial tissue,and differentially expressed proteins related to arrhythmia were screened.Key proteins were further identified through comparison with target proteins in databases combined with joint analyses.Bioinformatics methods were then used to investigate potential molecular mechanisms.Results A total of 356 differentially expressed proteins were identified,including 189 upregulated and 167 downregulated.Association analysis with target gene proteins identified 71 key proteins,and a protein-protein interaction network was constructed.GO enrichment and KEGG pathway analyses indicated that these key proteins were primarily involved in ion channel dysfunction,enhanced oxidative stress,and autonomic nervous system imbalance.Conclusion This study,through the integration of proteomics,data mining,and bioinformatics,revealed critical molecular mechanisms underlying SCD associated with lethal arrhythmia.These findings provide new perspectives and potential biomarkers for forensic identification and research on the mechanisms of death.
