Discussion on the Effects of Yanghe Decoction Containing Serum on Migration and Invasion of Breast Cancer Cells in Tumor Related Fat Microenvironment Based on the JAK2/STAT3 Signaling Pathway
10.19879/j.cnki.1005-5304.202404599
- VernacularTitle:基于JAK2/STAT3信号通路探讨阳和汤含药血清对肿瘤相关脂肪微环境中乳腺癌细胞迁移、侵袭的影响
- Author:
Jinlei LUO
1
;
Chengjie JIANG
;
Jianwei DOU
Author Information
1. 甘肃中医药大学基础医学院,甘肃省中药新产品创制工程实验室,甘肃省中医方药挖掘与创新转化重点实验室,甘肃 兰州 730000
- Publication Type:Journal Article
- Keywords:
Yanghe Decoction;
breast cancer;
tumor-associated adipocytes;
migration;
invasion;
BT-549 cells
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2025;32(3):105-111
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects and mechanism of Yanghe Decoction containing serum on the migration and invasion of BT-549 cells intervened by conditioned medium of cancer-associated adipocytes(CAAcm)based on the JAK2/STAT3 signaling pathway.Methods 3T3-L1 mouse embryonic fibroblasts were induced to differentiate into mature adipocytes using adipogenic induction medium.Yanghe Decoction containing serum was prepared from SD rats.BT-549 cells were co-cultured with mature adipocytes to obtain CAAcm.Transwell assay was used to detect the migration and invasion ability of BT-549 cells before and after intervention with CAAcm.BT-549 cells after CAAcm intervention were divided into blank serum group,positive drug group and Yanghe Decoction low-,medium-and high-dosage groups,and cultured with 10%corresponding serum.Cell proliferation was assessed using the CCK-8 assay,scratch assay was performed to evaluate the lateral migration of BT-549 cells,Transwell assay was conducted to evaluate the vertical migration and invasion ability of BT-549 cells,Western blot was performed to detect the expressions of proteins including JAK2,STAT3,VEGF and HIF-1α.Results CAAcm intervention had a promoting effect on the migration and invasion of BT-549 cells.Compared with the blank serum group,the growth inhibition rate of BT-549 cells significantly increased in Yanghe Decoction low-,medium-and high-dosage groups(P<0.05,P<0.01),the scratch healing rate was significantly decreased(P<0.05,P<0.01),the number of migrating cells and invading cells significantly reduced(P<0.01),and the expressions of JAK2,STAT3,VEGF and HIF-1α proteins significantly decreased(P<0.05,P<0.01).Conclusion Yanghe Decoction containing serum can inhibit the proliferation,migration and invasion of BT-549 cells intervened by CAAcm,and the mechanism may be related to the inhibition of JAK2,STAT3,VEGF and HIF-1α protein expressions.
