Identification of chemical components of Angelica sinensis using UPLC-Q-TOF/MS and its the effect and mechanism of activating blood circulation
- VernacularTitle:基于UPLC-Q-TOF/MS的当归化学成分鉴定及活血作用机制
- Author:
Wen-xing JIAO
1
;
Jiang-xia WEI
;
Jing-jing GUO
;
Zhi-jun YANG
;
Xi-cang YANG
;
Xiu-juan YANG
Author Information
- Publication Type:Journal Article
- Keywords: Angelica sinensis; chemical components; proteomics; acute blood stasis syndrome; effect of acti-vating blood circulation; mechanism of action
- From: Chinese Pharmacological Bulletin 2025;41(1):147-156
- CountryChina
- Language:Chinese
- Abstract: Aim To identify the chemical components of Angelica sinensis(AS)and explore the mechanism of AS in activating blood circulation.Methods UP-LC-Q-TOF-MS was used to identify the chemical com-ponents of AS.The changes of syndrome and patholog-ical section of heart in rats were observed.Hemody-namics and proteomics were measured.Results A to-tal of 270 compounds were identified from AS.It showed that rats of Angelica sinensis group were greatly improved such as arched back,shrugged fur,huddled up and less mobile,purplish paws and tails,whitish ear margins and nasolabial lips,reduced drinking and feed-ing,and slow response to external stimuli;mildly disor-dered myocardial fibre arrangement,myofibre arrange-ment was tighter than that of model group,myocardial fibres were narrower and close to normal,and mild oe-dema,exudation,and inflammatory cell infiltration could be seen in the surrounding area;SAP was signif-icantly lower and LVSP was significantly higher in An-gelica sinensis group(P＜0.05).Proteomics showed that 62 differential proteins were screened in Angelica sinensis group compared to model,GO function were concentrated in the extracellular matrix,cytoskeletal proteins binding and protein hydrolysis negatively regu-lated.KEGG pathway were enriched in signalling path-ways such as complement and coagulation cascades,cellular focal adhesion,leukocyte transendothelial mi-gration and chemokine signalling pathways.Conclu-sions AS probably through the expression of proteins,which modulate the signalling pathways of the comple-ment and coagulation cascade reactions and the con-traction of vascular smooth muscle.
