A study on comprehensive quality evaluation of six batches of Rhizoma Pinelliae for protective effects on chronic obstructive pulmonary disease based on TOPSIS method
10.12360/CPB202402005
- VernacularTitle:基于TOPSIS法综合评价法半夏抗慢性阻塞性肺疾病的品质研究
- Author:
Jun DENG
1
;
Xian-ju HUANG
;
Jun LI
;
Qi-en LI
;
Aikemu MAIHESUMU
;
Li-dan SHEN
Author Information
1. 中南民族大学药学院,湖北武汉 430074
- Publication Type:Journal Article
- Keywords:
Rhizoma Pinelliae;
Pinellia Ternata;
COPD;
Beas-2b cells;
TOPSIS;
cigarette smoke ex-tract
- From:
Chinese Pharmacological Bulletin
2025;41(1):138-146
- CountryChina
- Language:Chinese
-
Abstract:
Aim To evaluate the in vitro anti-chronic obstructive pulmonary disease(COPD)effects of six batches of Rhizoma Pinelliae(RP)ethanol extracts by the technique for order preference by similarity to an i-deal solution(TOPSIS),so as to establish a new method for comprehensive quality evaluation of RP.Methods Cigarette smoke extract(CSE)and lipopo-lysaccharide(LPS)were utilized to establish an airway inflammation model of Beas-2b human normal lung cells.MTT assay was employed to determine the toxici-ty and pharmacological concentrations of Pinellia Ter-nata(PT)and six different batches RP of their ethanol extracts.EC50 and IC50 values were calculated accord-ingly.Additionally,relevant markers were assessed u-sing qRT-PCR and the superoxide dismutase(SOD)assay kit.Finally,a comprehensive evaluation invol-ving multiple indices was performed using the TOPSIS method for both PT and the six batches of RP extracts.Results According to the IC50 values of seven differ-ent Pinellia ternata,the toxicity ranking was as fol-lows:PT＞RP④＞RP①＞RP⑤＞RP③＞RP⑥＞RP ②.On the other hand,the expressions of TNF-α,IL-6 and IL-8 were down-regulated in the treatment group compared with those of the model group by qRT-PCR.In addition,the levels of SOD de-creased in all groups after treatment.All the data were normalized and the comprehensive ranking results were obtained through TOPSIS analysis as follows:RP⑥＞RP⑤＞RP②＞RP①＞RP③＞RP④＞PT.Con-clusions The ethanol extracts of different Rhizoma Pinelliae varieties all demonstrate the capacity to sup-press inflammation and oxidative stress induced by LPS and CSE.The comprehensive evaluation indicates that the quality of the ⑥ batch of RP shows the highest quality.Moreover,the TOPSIS method can serve as an assessment approach for medicinal materials,offering a pathway for controling the varying quality of herbal ma-terials available in the market.
