Senecio scandens Buch.-Ham alleviates inflammatory pain through inhibiting mast cell P2X7 receptors
- VernacularTitle:千里光通过抑制肥大细胞P2X7受体缓解炎性疼痛
- Author:
Fan YE
1
;
Li-hui LIAO
;
Jing-yue TANG
;
Jia-hui CHEN
;
Jiang CHENG
;
Gong-xi CHEN
;
Zong-xiang TANG
Author Information
- Publication Type:Journal Article
- Keywords: Senecio scandens Buch.-Ham; Pain; Mast cell; P2X7 receptor; inflammatory; ATP
- From: Chinese Pharmacological Bulletin 2025;41(1):131-137
- CountryChina
- Language:Chinese
- Abstract: Aim To investigate the effect of Senecio scandens Buch.-Ham on inflammatory pain mediated by mast cell P2X7 receptor.Methods Using the ATP-induced foot inflammatory pain,immunofluores-cence and toluidine blue staining techniques were used to investigate whether Senecio scandens has inhibitory effect on P2X7 receptor on mast cells.Using the calci-um ion imaging experimental technology,to explore whether Senecio scandens inhibit the intracellular cal-cium ion enrichment caused by activation of P2X7 re-ceptor on mouse peritoneal mast cell.The whole-cell patch clamp technique was used to investigate whether senecio scandens could inhibit the inward current in-duced by activation of P2X7 receptor on mouse perito-neal mast cell.Results In vivo,Senecio scandens alle-viate ATP induced inflammatory pain(3.9 g·kg-1:P＜0.05),and significantly inhibited the infiltration of P2X7 receptor-positive mast cells(3.9 g·kg-1:P＜0.05).Knockout of mast cell can reduce the analgesic effect of Senecio scandens(3.9 g·kg-1:P=0.645).In vitro.The experiment results show that senecio scandens can significantly inhibit the calcium influx(300 mg·L-1:P＜0.05;1 g·L-1:P＜0.01;3 g·L-1:P＜0.01)and the inward current mediated by P2X7 receptor in mast cell(1 g·L-1:P＜0.01).Conclusion Senecio scandens alleviate inflammatory pain by inhibiting mast cell P2X7 receptor.
