Formononetin inhibits formation of Staphylococcus epidermidis biofilm by targeting sortase A
10.13602/j.cnki.jcls.2025.09.10
- VernacularTitle:芒柄花黄素靶向分选酶A抑制表皮葡萄球菌生物膜的形成
- Author:
Kai ZHANG
1
;
Xiaoxiao LIU
;
Feifei YANG
;
Bing GU
Author Information
1. 徐州医科大学附属徐州市立医院医学检验科,江苏 徐州 221116;徐州医科大学医学技术学院,江苏 徐州 221004
- Publication Type:Journal Article
- Keywords:
formononetin;
Staphylococcus epidermidis;
sortase A;
biofilm
- From:
Chinese Journal of Clinical Laboratory Science
2025;43(9):689-694
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the inhibitory effects of formononetin on sortase A(SrtA)of Staphylococcus epidermidis(S.epi-dermidis)and its biofilm formation.Methods Biofilm-producing S.epidermidis standard strain RP62A and biofilm-producing S.epider-midis clinical strains were collected.The minimal inhibitory concentration(MIC)of formononetin against S.epidermidis was investigated using the two-fold dilution method.The inhibitory effects of different concentrations of formononetin on the growth of S.epidermidis in vitro were studied by growth curve analysis using microplate reader.The cytotoxicity of formononetin was measured by cell culture.The biocompatibility of formononetin was studied by hemolysis assay.The inhibitory effect of formononetin on the activity of SrtA was deter-mined by fluorescence resonance energy transfer(FRET)assay.The effect of formononetin on the ability of S.epidermidis binding fi-bronectin was assessed by fibronectin binding assay.The effect of formononetin on the anchoring of serine-rich repeat proteins(SRRPs)in S.epidermidis was assessed by immunofluorescence staining.The effect of formononetin on the biofilm formation of S.epidermidis was measured by crystal violet staining.Results The MICs of formononetin against S.epidermidis were greater than 512 μg/mL.Compared with the control group,32 μg/mL of formononetin did not significantly affect the growth of S.epidermidis or reduce the viability of hu-man aortic endothelial cells.The hemolysis rate of human red blood cells was less than 5%.The inhibition rate of SrtA activity was ap-proximately 53.8%.The binding capacity of S.epidermidis to fibronectin was reduced by approximately 45.4%.Microscopic observation revealed a significant reduction in the anchoring of SRRP in S.epidermidis,and the formation of biofilm of S.epidermidis was inhibited by approximately 41.7%.Conclusion Formononetin inhibits the activity of SrtA in S.epidermidis,reduce its fibronectin-binding ca-pacity and biofilm formation,and thus represents a promising candidate for the prevention of S.epidermidis infections.
