The protective effect of methyl rosmarinate on myocardial injury induced by high altitude hypoxia and its network pharmacology study
- VernacularTitle:迷迭香酸甲酯对高原缺氧心肌损伤的保护作用和网络药理学研究
- Author:
Qian JI
1
;
Yue-mei SUN
;
Fang-fang CHOU
;
Yan-ling WANG
;
Rong WANG
;
Wen-bin LI
Author Information
- Publication Type:Journal Article
- Keywords: methyl rosemarinate; plateau; hypoxic myocardial injury; network pharmacology; molecular docking; oxidative stress
- From: Chinese Pharmacological Bulletin 2025;41(10):1956-1962
- CountryChina
- Language:Chinese
- Abstract: Aim To investigate the protective effects of methyl rosmarinate(MR)on myocardial injury in-duced by high-altitude hypoxia and explore its underly-ing mechanisms.Methods BALB/c mice were ran-domly divided into a control group,a model group,and low-,medium-,and high-dose MR groups(25,50,and 75 mg·kg-1,respectively).Except for the control group,all other groups were exposed to a hypobaric hypoxia chamber and administered MR via intraperitoneal injection daily for three days.After the experiment,myocardial tissues were collected for he-matoxylin and eosin(HE)staining to observe morpho-logical changes.Levels of malondialdehyde(MDA),glutathione(GSH),and superoxide dismutase(SOD)were measured to evaluate the anti-myocardial injury activity of MR.Network pharmacology was employed to predict drug-disease interaction targets,construct a protein-protein interaction(PPI)network,and identify core targets.Functional enrichment analysis was car-ried out using Gene Ontology(GO)and Kyoto Ency-clopedia of Genes and Genomes(KEGG)pathways.Molecular docking was used to verify the binding affini-ty of MR to core targets,and Western blot was conduc-ted to detect the expression of related proteins.Results MR significantly alleviated myocardial injury caused by high-altitude hypoxia.Network pharmacology analy-sis identified EGFR,Bcl-2,STAT3,MMP9,ESR1,and MTOR as key targets.Molecular docking con-firmed strong binding between MR and these core tar-gets.Western blot results demonstrated that MR im-proved myocardial injury by regulating the expression of STAT3,Bax,and Bcl-2 proteins.Conclusion MR may exert its protective effects on high-altitude hypoxi-a-induced myocardial injury through a multi-target mechanism.
