Effect of STAT3 on PHEV replication in hippocampal neuronal cells
- VernacularTitle:STAT3在海马神经元细胞中对PHEV复制的影响
- Author:
Yubo JIAO
1
;
Zhenzhen WANG
1
;
Qinyu WANG
1
;
Hanzhe ZHOU
1
;
Sirui LI
1
;
Wenqi HE
1
;
Feng GAO
1
;
Yungang LAN
1
Author Information
- Publication Type:Journal Article
- Keywords: PHEV; HT22 cells; STAT3; virus replication
- From: Chinese Journal of Veterinary Science 2025;45(7):1366-1371,1393
- CountryChina
- Language:Chinese
- Abstract: In order to explore the role of signal transducer and activator of transcription 3(STAT3)in the infection process of porcine hemagglutinating encephalomyelitis virus(PHEV),Western blot,qRT-PCR and indirect immunofluorescence experiments were used to detect the phosphoryla-tion level and subcellular localization changes of STAT3 after PHEV infection.The replication of PHEV were examined in cells with STAT3 knockdown or overexpression,respectively.The results showed the phosphorylation level of STAT3 at tyrosine 705 was significantly increased after PHEV infection,and the expression of STAT3 in the nucleus increased.In addition,STAT3 knock-down in cells can significantly inhibit PHEV replication.The above results further reveal the path-ogenic mechanism of PHEV and provide a theoretical basis for the research of anti-PHEV drugs.
