Effects of Wuling Powder on Mice with Hyperlipidemia
10.16466/j.issn1005-5509.2024.12.002
- VernacularTitle:五苓散抗高脂饮食诱导的高脂血症作用研究
- Author:
Yanhua ZHANG
1
;
Ke LI
;
Weizhi LAI
Author Information
1. 浙江中医药大学药学院 杭州 310053;浙江中医药大学附属第一医院(浙江省中医院)
- Publication Type:Journal Article
- Keywords:
Wuling powder;
hyperlipidemia;
TC;
TG;
CETP;
PLTP;
TBA;
CYP7A1
- From:
Journal of Zhejiang Chinese Medical University
2024;48(12):1482-1491
- CountryChina
- Language:Chinese
-
Abstract:
[Objective]To investigate the protective effect of Wuling Powder on hyperlipidemic(HLP) mice and the underlying mechanism.[Methods]Forty-eight male ICR mice were randomly divided into six groups:normal,model,Ezetimibe and Wuling Powder low,middle,high dose groups. Mice in all groups were treated with a high-fat diet for 8 weeks,except for normal group. Meanwhile,the mice in drug intervention groups were treated with Ezetimibe or Wuling Powder. General physical signs of mice in each group were observed and recorded,total cholesterol(TC),triglyceride(TG),high density lipoprotein cholesterol(HDL-C),low density lipoprotein cholesterol(LDL-C) and total bile acid(TBA) were detected. Liver lipid deposition was detected by hematoxylin-eosin(HE) staining and oil red staining. Cholesterol ester transfer protein(CETP) and recombinant phospholipid transfer protein(PLTP) was detected with enzyme-linked immunosorbent assay(ELISA). Immunohistochemistry was used to detect the expression of cholesterol 7α-hydroxylase(CYP7A1) in the liver. Ultra-high performance liquid chromatograph coupled triple quadrupole mass spectrometer(UPLC-MS/MS) was applied to measure bile acid(BAs) levels in liver tissue. Expression of intestine Takeda G protein-coupled receptor 5(TGR5) and farnesoid X receptor(FXR1) was detected with fluorescence analysis.[Results]Wuling Powder could inhibit the increase in body weight of mice induced by high-fat diet. In the 4th week,low dose group of Wuling Powder reduced TC level(P<0.01),each group of Wuling Powder reduced the level of TG(P<0.01);in the 8th week,high dose of Wuling Powder could increase the level of TG(P<0.05),there was no statistical significance in the level of TC,HDL-C and LDL-C(P>0.05). Wuling Powder could reduce hepatic cell fat vacuoles and number of lipid droplets;middle and high doses of Wuling Powder could increase the level of PLTP(P<0.01),low and high doses of Wuling Powder could increase the level of CETP(P<0.05,P<0.01),reduce serum level of TBA,and increase immunohistochemical detection of CYP7A1 expression in the liver(P<0.01),and restore disorders in BAs metabilism,at the same time,simultaneously reducing the levels of TGR5 and FXR1 in the intestine.[Conclusion]Wuling Powder can reduce the level of blood lipids in HLP mice,and its mechanism may be related to promote the reverse transport of cholesterol by increasing the levels of CETP and PLTP,and regulate liver BAs metabolism balance of HLP mice.
