BHB inhibits phagocytic function of CD14+monocytes in dairy cows via ROS-NL-RP3 pathway
10.16303/j.cnki.1005-4545.2025.07.18
- VernacularTitle:BHB通过ROS-NLRP3途径抑制奶牛CD14+单核细胞吞噬功能
- Author:
Wantong CHENG
1
;
Yang CHI
;
Xudong SUN
;
Chuang XU
Author Information
1. 黑龙江八一农垦大学 动物科技学院,黑龙江 大庆 163319
- Publication Type:Journal Article
- Keywords:
BHB;
NLRP3 inflammasome;
CD14+monocyte;
phagocytosis;
dairy cow
- From:
Chinese Journal of Veterinary Science
2025;45(7):1493-1501
- CountryChina
- Language:Chinese
-
Abstract:
The aim of this study was to investigate whether β-hydroxybutyric acid(BHB)inhibits the phagocytic function of CD14+monocytes in dairy cows through the ROS(reactive oxygen spe-cies)-NLRP3(NOD-like receptor thermal protein domain associated protein 3)pathway.CD14+monocytes in the blood of postpartum healthy cows were extracted,and 3 mmol/L BHB was added after transfection of small interfering RNA targeting NLRP3(si-NLRP3).Monocytes were pre-treated with 10 nmol/L NLRP3 inhibitor MCC950(CP-456773)or 10 mmol/L ROS scavenger N-acetyl cysteine(NAC),and then was treated with 3 mmol/L BHB for 24 h.The protein abundance of NLRP3 was detected by Western blot and the phagocytosis of monocytes was determined by flow cytometry and immunofluorescence.The results showed that compared with the si-Control+BHB group,the phagocytic function of monocytes in the si-NLRP3+BHB treatment group was significantly increased,while the protein abundance of NLRP3 was significantly decreased.Com-pared with DMSO+BHB group,the phagocytic function of monocytes in MCC950+BHB group was significantly increased.In addition,compared with DMSO+BHB group,the protein abundance of NLRP3 in monocytes was significantly decreased in MCC950+BHB group.Compared with the PBS+BHB group,the phagocytosis of monocytes was significantly increased after the addition of ROS scavenger NAC,while the protein abundance of NLRP3 was significantly decreased.These results indicated that BHB inhibited the phagocytosis of CD14+monocytes in cows via the ROS-NLRP3 pathway.Therefore,regulating the activation of NLRP3 inflammasome may be an effective method to improve the decrease of monocyte phagocytosis in perinatal dairy cows.
