Immunomodulatory effect of E515 on rabbit peripheral blood lymphocytes and-Bordetella bronchiseptica inactivated vaccine
10.16303/j.cnki.1005-4545.2025.07.11
- VernacularTitle:E515对兔外周血淋巴细胞和波氏杆菌灭活疫苗的免疫调节作用
- Author:
Xuemei CUI
1
;
Qiuju XIANG
;
Ye'e HUANG
;
Quanan JI
;
Tuanyuan SHI
;
Zizhe HU
;
Guo-lian BAO
;
Yan LIU
Author Information
1. 浙江省农业科学院畜牧兽医研究所,浙江 杭州 310021
- Publication Type:Journal Article
- Keywords:
E515 adjuvant;
peripheral blood lymphocyte;
Bordetella bronchiseptica inactivated vac-cine;
immunomodulation
- From:
Chinese Journal of Veterinary Science
2025;45(7):1437-1442,1492
- CountryChina
- Language:Chinese
-
Abstract:
To explore the immunomodulatory effects of a new vegetable oil adjuvant(named E515)containing vitamin E(VE)and ginsenosides(GS)on rabbit peripheral blood lymphocytes and Bordetella of rabbit inactivated vaccine.E515,Bordetella bronchiseptica(Bb)and LPS were co-cultured with rabbit peripheral blood lymphocytes in vitro,and the lymphocyte conversion rate was detected by CCK8 method,and the content of lymphocyte supernatant cytokines was detected by ELISA method.After rabbits were immunized with E515-Bb vaccine,the antibody level was detec-ted by indirect ELISA,the serum cytokine content was detected by ELISA,and the protective effect of E515-Bb vaccine on rabbits was observed by challenge test.In vitro cell experiments showed that E515 could significantly increase lymphocyte proliferation and TH1/TH2 cytokine se-cretion in rabbit peripheral blood.In vivo animal experiments showed that E515 adjuvant could sig-nificantly enhance the level of Bb specific antibody induced by Bordetella vaccine in rabbits.In-crease the secretion level of TH1/TH2 cytokines and decrease the secretion level of TNF-α;It can effectively protect rabbits against Bordetella infection with a protection rate of 91.67％.Therefore,E515 as a new vegetable oil adjuvant deserves further study.
