Isolation of Endophytic Fungi from Glycyrrhiza glabra L.and Study of Their Secondary Metabolite Activity
- VernacularTitle:光果甘草内生真菌的分离及其次级代谢产物活性研究
- Author:
Yang LI
1
;
Zhipeng JIA
1
;
Yujie ZHAO
1
;
Xiaoxiao ZHAO
1
;
Jiamei PEI
1
;
Kailin CHEN
1
;
Zaiping FENG
1
;
Xinguo ZHANG
1
Author Information
- Publication Type:Journal Article
- Keywords: Glycyrrhiza glabra L.; Endophytic fungi; Isolation; Identification; Secondary metabolites; Enzyme inhibition activity
- From: World Science and Technology-Modernization of Traditional Chinese Medicine 2024;26(11):2996-3005
- CountryChina
- Language:Chinese
- Abstract: Objective Endophytic fungi were isolated and purified from fresh Glycyrrhiza glabra L.and strains capable of producing secondary metabolites with inhibitory effects on adenosine deaminase,β-lactamase,xanthine oxidase,and tyrosinase were screened.Methods Four types of culture media were used to isolate and purify endophytic fungi using tissue homogenization method.Subsequently,the secondary metabolites of endophytic fungi were evaluated using an activity assessment platform.Results A total of 131 strains of endophytic fungi were isolated from the plant,among which 34 strains exhibited distinct morphologies and special characteristics.The mycelial extract of hypha G(1-5)(s)showed good adenosine deaminase inhibitory activity(inhibition rate:98.58％)and β-lactamase inhibitory activity(inhibition rate:93.15％).and β-lactamase(inhibition rate:93.15％).Strain G6 exhibited excellent xanthine oxidase inhibitory activity(inhibition rate:91.38％)in the ethyl acetate phase.These strains were identified as Penicillium sp.and Aspergillus sp.Conclusion Glycyrrhiza glabra L.harbors a diverse range of endophytic fungi with promising research potential.Further investigation is warranted to provide important technical data for the protection and in-depth applications of Glycyrrhiza glabra L..
