Yiqi Wenfei Tongqiao decoction alleviates inflammatory response in rats with allergic rhinitis of lung qi deficiency and cold type by regulating JAK1/STAT3 signaling pathway
10.12007/j.issn.0258-4646.2025.09.011
- VernacularTitle:益气温肺通窍方通过调节JAK1/STAT3信号通路减轻肺气虚寒型变应性鼻炎大鼠炎症反应
- Author:
Yijin ZHOU
1
;
Zhipeng ZHU
1
;
Xinlei TIAN
1
;
Wenjin ZHAO
1
;
Shan ZHU
1
Author Information
1. 河南省中医院(河南中医药大学第二附属医院)儿科,郑州 450053
- Publication Type:Journal Article
- Keywords:
Yiqi Wenfei Tongqiao decoction;
allergic rhinitis of lung qi deficiency and cold type;
inflammatory response;
Janus kinase 1;
signal transducer and activator of transcription 3
- From:
Journal of China Medical University
2025;54(9):826-831
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of Yiqi Wenfei Tongqiao decoction on the inflammatory response in rats with allergic rhi-nitis(AR)of lung qi deficiency and cold type based on the Janus kinase 1(JAK1)/signal transducer and activator of transcription 3(STAT3)signaling pathway.Methods A AR model of lung qi deficiency and cold type was established in rats using ovalbumin(OVA)combined with smoking and cold stimulation.After modeling,the rats were randomly divided into model,loratadine,low-dose Chinese medicine,high-dose Chinese medicine,and high-dose Chinese medicine+RO8191(JAK1/STAT3 signaling pathway activator)groups,with 12 rats per group.Twelve healthy rats were used as controls.After 14 days of drug intervention,the changes in the symptoms of lung qi deficiency and cold syndrome and AR were observed in the rats.Enzyme-linked immunosorbent assay was used to detect the serum levels of tumor necrosis factor α(TNF-α),interleukin(IL)-4,interferon-γ(IFN--γ),and ovalbumin-specific immunoglobulin E(OVA-sIgE).The number of inflammatory cells in the nasal lavage fluid was determined and the eosinophil(EOS)percentage was calculated.Pathological changes in the nasal mucosal tissue were observed after hematoxylin and eosin(HE)staining.Western blotting was used to detect the expression of JAK1/STAT3 signaling pathway-related proteins in nasal mucosal tissue.Results Compared with those in the model group,the AR symptom score,serum IL-4,TNF-α,and OVA-sIgE levels,EOS percentage in nasal lavage fluid,and levels of p-JAK1/JAK1 and p-STAT3/STAT3 in the nasal mucosal tissue of rats in the loratadine,low-dose Chinese medicine,and high-dose Chinese medicine groups were lower,whereas the serum IFN-γ level was higher(P＜0.05).RO8191 weakened the inhibitory effect of Yiqi Wenfei Tongqiao decoction on the inflammatory response in rats with AR of lung qi deficiency and cold type.Conclusion Overall,Yiqi Wenfei Tongqiao decoction can alleviate the inflammatory response in rats with AR of lung qi deficiency and cold type by inhibiting JAK1/STAT3 signaling pathway acti-vation and regulating the balance of Th1/Th2 cytokines.
