Effects of phloretin on the proliferation,apoptosis,and tumorigenicity of ovarian cancer cells by regulating Rac1/Akt/NF-κB signaling pathway

Yuehong TANG; Yuning LUO; Libin XU; Xiaoying HU; Chunping QIU

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Effects of phloretin on the proliferation,apoptosis,and tumorigenicity of ovarian cancer cells by regulating Rac1/Akt/NF-κB signaling pathway

VernacularTitle:根皮素通过调节Rac1/Akt/NF-κB信号通路对卵巢癌细胞增殖、凋亡和致瘤性的影响
Author: Yuehong TANG ¹ ; Yuning LUO ¹ ; Libin XU ¹ ; Xiaoying HU ¹ ; Chunping QIU ¹
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1. 海南省妇产科医院妇产科,海口 570100
Publication Type:Journal Article
Keywords: phloretin; Rac1/Akt/NF-κB signaling pathway; proliferation; tumorigenicity; apoptosis
From: Journal of China Medical University 2025;54(9):821-825,831
CountryChina
Language:Chinese
Abstract: Objective To explore the effect of phloretin on the proliferation,apoptosis,and tumorigenicity of ovarian cancer cells by regu-lating the Rac1/Akt/NF-κB signaling pathway.Methods The ovarian cancer cell line SKOV3 and the human normal ovarian epithelial cell line IOSE-80 were divided into the following groups:control,low-dose phloretin,medium-dose phloretin,high-dose phloretin,PM A,and high-dose phloretin+PMA.Morphological changes were observed under a microscope.Cell viability and apoptosis were assessed using the CCK-8 assay,colony formation assay,and flow cytometry.Western blotting was performed to detect the expression of proteins related to the Rac1/Akt/NF-κB signaling pathway.Tumor-bearing nude mice were established,tumor weights were measured,and the expres-sion levels of Rae1 and Akt in tumor tissues were analyzed.Results Compared with the control group,SKOV3 cells treated with low-,medium-,and high-dose phloretin showed reduced survival rate,colony formation,and expression of p-NF-κB p65/NF-κB p65,p-Akt/Akt,and Rac 1 in a dose-dependent manner.However,PM A reversed the inhibitory effects of high-dose phloretin on the malignant progression of ovarian cancer.In vivo experiments demonstrated that phloretin significantly inhibited tumor growth and reduced Akt and Rae1 expres-sion in tumor tissues(P＜0.05).Conclusion Phloretin suppresses the malignant progression of ovarian cancer by inhibiting the Rae1/Akt/NF-κB signaling pathway.