Optimization of"Honghuangbai"Gel Extraction Process and Establishment of Content Determination Method
10.3870/j.issn.1004-0781.2025.08.020
- VernacularTitle:"红黄白"凝胶提取工艺优选与含量测定方法的建立
- Author:
Yanguo GAO
1
;
Haitao ZHU
;
Liangyong HUANG
;
Fang YE
;
Qibin WANG
;
Tao ZHENG
;
Li CHEN
Author Information
1. 湖北医药学院附属太和医院药学部,十堰 442000;湖北医药学院附属太和医院武当中医药研究所,十堰 442000;湖北医药学院药学院,十堰 442000
- Publication Type:Journal Article
- Keywords:
Honghuangbai gel;
Extraction process;
AHP-CRITIC composite weighted;
Orthogonal test;
Content determination
- From:
Herald of Medicine
2025;44(8):1322-1329
- CountryChina
- Language:Chinese
-
Abstract:
Objective To optimized the extraction process of the"Honghuangbai"gel and to establish the content determination method of the gel.Methods The contents of salidroside,berberine hydrochloride and 1,4-bis[4-(glucose-oxy)benzyl]-2-isobutyl malate in"honghuangbai"extract were determined by HPLC with gradient elution;the content of total polysaccharide was determined by anthrone-sulfuric acid method.AHP-CRITIC composite weighting method was used to calculate the weight coefficient of 4 indicators,and the comprehensive score was taken as the evaluation index.Combined with L9(34)orthogonal experiment design,the effects of solid-liquid ratio,extraction time and times on the extraction process of"honghuangbai"gel were investigated,and the optimal extraction process was selected and verified.The method for the determination of 4 index components in the gel was established and the methodology was investigated.Results The optimal solid-liquid ratio was 1∶45,the optimal extraction time was 2 h and the optimal extraction times was twice.The linear relationships of the four indexes were good in their respective linear ranges,with R2＞0.999 0.The average recoveries were 98.67％～102.09％,and RSD was 0.77％～3.17％.Good precision,repeatability and stability,RSD≤2.28％.Conclusion The optimal extraction process of"honghuangbai"gel optimized by AHP-CRITIC combined with orthogonal test is stable and economical.The method of the gel content determination is feasible and the result is reliable,which provides experimental basis for the establishment of the quality standard of the gel.
