Antioxidative effects of NF-E2-related factor 2 on acute lung injury caused by seawater drowning in mice
10.3760/cma.j.cn311847-20191012-00253
- VernacularTitle:Nrf2对小鼠海水淹溺肺损伤氧化应激的作用研究
- Author:
Yubao QIU
1
;
Jing ZUO
1
;
Mingxia WEI
1
;
Can YANG
1
;
Jinhong FAN
1
;
Yan CHEN
1
;
Yaxian WU
1
;
Qingfeng PANG
1
Author Information
1. 214122 江苏省无锡，江南大学无锡医学院
- Publication Type:Journal Article
- Keywords:
NF-E2-related factor 2;
Seawater drowning;
Lung injury;
Oxidative stress
- From:
Chinese journal of nautical medicine and hyperbaric medicine
2020;27(2):152-156
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the role of NF-E2-related factor 2 (Nrf2) in lung injury caused by seawater drowning in mice.Methods:A total of 48 6-8 weeks old male wild-type C57 mice were randomly divided into two groups for experiment: (1) control group, day 1 group after drowning(Day1), day 3 group after drowning(Day3) and day 7 group after drowning(Day7) to determine the time point of seawater drowning; (2) control group, seawater drowning model group(SW), dimethyl fumarate treatment group(DMF) and seawater drowning+ DMF reatment group(SW+ DMF) to determine the role of Nrf2 in lung injury in mice caused by drowning, 6 mice per group. And 12 male C57 background Nrf2 knockout mice (Nrf2 KO) at 6-8 weeks old were divided into Nrf2 KO control group and Nrf2 KO+ seawater drowning group; 6 mice in each group. Three days after the drowning model was established, we observed the gross morphology and the pathological changes of the lung tissue, calculated the wet-to-dry weight ratio of the lungs, and tested the reduced glutathione and malondialdehyde levels.Results:Seawater drowning caused alveolar space rupture, pulmonary hemorrhage and pulmonary edema in mice. Compared with the wild-type mice, knockout of Nrf2 aggravated lung injury and oxidative stress in mice caused by seawater drowning. Nrf2 agonist DMF treatment significantly improved lung injury in mice, increased the reduced glutathione content, and decreased malondialdehyde level.Conclusion:The activation of Nrf2 can relieve lung injury and oxidative stress levels in mice caused by seawater drowning, which can play an important role in reducing lung damage caused by seawater drowning.
