Effect of different oxygen partial pressure on the survival of PC12 cells in rats
10.3760/cma.j.cn311847-20190128-00014
- VernacularTitle:不同氧分压对大鼠PC12细胞生存的影响
- Author:
Qiuhong YU
1
;
Yaling LIU
1
;
Cong WANG
1
;
Caixia QIU
1
;
Lianbi XUE
1
Author Information
1. 100070 北京，首都医科大学附属北京天坛医院高压氧科
- Publication Type:Journal Article
- Keywords:
Oxygen partial pressure;
Hyperbaric oxygen;
Reactive oxygen species;
Mitochondrial membrane potential;
Apoptosis
- From:
Chinese journal of nautical medicine and hyperbaric medicine
2020;27(2):239-243
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To observe the effect of different oxygen partial pressure on the survival of adrenal medullary pheochromocytoma (PC12) cells in rats and explore the possible mechanism.Methods:When carpeting 70%-80% of the entire flask bottom, the PC12 cells were randomly divided into 0.1 MPa group, 0.2 MPa group, and 0.4 MPa group. Then each group was placed in the experiment chamber respectively. The 0.1 MPa group was given pure oxygen under normal pressure in which oxygen partial was 0.1 MPa; while the other two groups was given 0.2 MPa and 0.4 MPa partial pure oxygen respectively with 0.03 MPa/min compresing and decompressing. Pure oxygen was given for 1 h under the corresponding stable pressure. Cell viability and the level of L-lactate dehydrogenase (LDH) in cultrue solution were determined by 3-(4, 5-dimethylthiazol-2yl)-2, 5-diphenyl tetrazolium bromide (MTT) colorimetric assay, intracellular reactive oxygen species (ROS) and mitochondrial membrane potential (MMP) were detected by flow cytometry.Results:(1) The cell viability in the 0.2 MPa group (118.35±5.42)% was significantly higher than that in the 0.1 MPa group (100.38±5.08)% ( P<0.01), while the cell viability in the 0.4 MPa group (83.50±7.11)% was lower than those in the 0.1 MPa group and the 0.2 MPa group ( P<0.01). (2) The level of LDH in the 0.4 MPa group (1 071.67±35.36) was significantly increased, which was significantly different from those in the 0.1 MPa group (959.19±34.06) and the 0.2 MPa group (966.66±31.38) ( P<0.01). (3) The level of ROS rised as the oxygen partial pressure increased. The differences of ROS level between the groups, i. e. the 0.1 MPa group (97.48±6.08) and the 0.2 MPa group (112.48±3.14), the 0.1 MPa group and the 0.4 MPa group (148.62±4.79), and the 0.2 MPa group and the 0.4 MPa group, were all statistically significant ( P<0.01). (4) The fluorescence intensity of Rh123 in each group were (797.63±60.05), (798.20±58.54), and (1 362.32±40.68) respectively, which were negatively correlated with MMP. There was no significant difference in MMP between the 0.1 MPa group and the 0.2 MPa group ( P=0.79), but the MMP in the 0.4 MPa group was lower than those in 0.1 group and the 0.2 MPa group with statistical significance ( P<0.01). Conclusion:The appropriate oxygen partial pressure could increase the viability of PC12 cells while a high oxygen partial pressure may be toxic, produce excessive ROS, and then induce the decrease of MMP, which is one of the possible mechanisms.
