Effects of hyperbaric oxygen therapy on differentiation of endogenous neural stem cells in rats with focal cerebral ischemia at different stages
10.3760/cma.j.cn311847-20190918-00228
- VernacularTitle:造模后不同时间高压氧治疗对局灶性脑缺血大鼠内源性神经干细胞分化的影响
- Author:
Yongjun MAI
1
;
Xiang LI
;
Liangzuo ZHENG
;
Hong YANG
;
Xiaomou WEI
;
Lianbi XUE
Author Information
1. 545005 广西壮族自治区柳州，广西医科大学第四附属医院
- Publication Type:Journal Article
- Keywords:
Hyperbaric oxygen;
Cerebral ischemia;
Neural stem cells;
Rats;
Animal model
- From:
Chinese journal of nautical medicine and hyperbaric medicine
2020;27(2):224-229
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To observe the differentiation of endogenous neural stem cells (NSCs) in the ischemic brain of rats with focal cerebral ischemia at different stages with various treatments.Methods:The model of focal cerebral ischemia was established by middle cerebral artery occlusion (MCAO). A total of 105 rats were randomly divided into sham-surgery group (SS), ischemia-reperfusion group (IR), receiving hyperbaric oxygen (HBO) 1 day after modeling group (IR1d+ HBO), receiving HBO 7 days after modeling group (IR7d+ HBO group), receiving HBO 14 days after modeling group (IR14d+ HBO group), and receiving HBO 28 days after modeling group (IR28d+ HBO group). At the 2nd, 4th, 6th and 8th week after modeling, the cells of newly generated neurons and astrocyte in the ischemic area were positive in both immunofluorescence BrdU/β-tubulin and BrdU/glial fibrillary acidic protein (GFAP) test were detected.Results:Two weeks after modeling, the expressions of BrdU/β-tubulin were 11.40±1.52 in the SS group, 36.60±2.51 in the IR group, 48.60±3.21 in the IR1d+ HBO group, and 42.60±2.30 in the IR7d+ HBO group; four weeks after modeling, it was 12.20±1.92 in the SS group, 26.20±2.28 in the IR group, 39.80±2.17 in the IR1d+ HBO group, 33.20±1.92 in the IR7d+ HBO group, 29.20±1.48 in the IR14d+ HBO group. Six weeks after modeling, it was 12.40±1.67 in the SS group, 18.60±1.82 in the IR group, 30.20±2.49 in the IR1d+ HBO Group, 25.40±2.79 in the IR7d+ HBO group, 21.40±2.50 in the IR14d+ HBO group, 19.60±1.67 in the IR28d+ HBO group. Eight weeks after modeling, it was 11.80±1.64 in the SS group, 16.40±2.07 in the IR group, 24.40±1.95 in the IR1d+ HBO group, 21.20±1.48 in the IR7d+ HBO group, 18.40±1.67 in the IR14d+ HBO group, and 15.60±1.82 in the IR28d+ HBO group. The differences in the expressions of BrdU/β-tubulin between these groups were statistically significant ( P<0.01). Compared with the SS group, the expression of BrdU/β-tubulin increased in the IR group at each time point ( P<0.05). The expressions of BrdU/β-tubulin in the IR1d+ HBO and the IR7d+ HBO were higher than those in the IR group at each time point; while there were statistical differences comparing those in the IR14d+ HBO group and the IR28d+ HBO group with those in the IR group at each time point ( P>0.05). The expressions of BrdU/β-tubulin were at the highest level at the second week in all the groups except the SS group ( P<0.05). (2) Two weeks after modeling, the expression of BrdU/GFAP was 22.60±1.82 in the SS group, 59.00±3.67 in the IR group, 50.60±2.51 in the IR1d+ HBO group, and 55.20±2.58 in the IR7d+ HBO group. Four weeks after modeling, it was 22.20±1.48 in the SS group, 45.00±2.24 in the IR group, 35.80±1.64 in the IR1d+ HBO group, 40.20±2.16 in the IR7d+ HBO group, and 44.60±2.51 in the IR14d+ HBO group. Six weeks after modeling, it was 22.80±1.64 in the SS group, 26.68±1.78 in the IR group, 27.40±1.67 in the IR1d+ HBO group, 28.40±1.51 in the IR7d+ HBO group, 26.20±1.78 in the IR14d+ HBO group, and 26.20±1.48 in the IR28d+ HBO group. Eight weeks after modeling, it was 21.60±1.81 in the SS group, 21.40±1.14 in the IR group, 24.00±1.58 in the IR1d+ HBO group, 24.80±1.92 in the IR7d+ HBO group, 23.40±1.67 in the IR14d+ HBO group, and 22.20±1.30 in the IR28d+ HBO group. At each time point, the differences of BrdU/GFAP expression between each group were statistically significant ( P<0.05). Furthermore, the BrdU/GFAP expression in the IR group was higher than that in the SS group at each time point except the 8th week. The expressions of BrdU/GFAP in the IR1d+ HBO group and the IR7d+ HBO group were higher than those in the IR group at the 2nd and the 4th weeks ( P<0.05). There was no statistically significant difference in the expressions of BrdU/GFAP between the IR1d+ HBO group and the IR7d+ HBO group at the 6th and the 8th week( P>0.05). There was no statistically significance comparing the expressions of BrdU/GFAP in the IR14d+ HBO and the IR 28d+ HBO group respectively with those in the SS group( P>0.05) at each time point. All the expressions of BrdU/GFAP in each group reached the highest level at the 2nd week ( P<0.05). Conclusion:In the early and the recovery period, HBO can promote the differentiation of NSCs in the ischemic areas into neurons and inhibit its differentiation into astrocytes. An early long-term treatment of HBO can maintain NSCs differentiation in the ischemic area into neurons for a long time.
