Honokiol inhibits the malignant progression of pancreatic cancer cells by regulating glycolysis through the Raf/MEK/ERK pathway
- VernacularTitle:和厚朴酚通过Raf/MEK/ERK通路调控糖酵解抑制胰腺癌细胞恶性进展
- Author:
Xin LIU
1
;
Jing LI
;
Xi WANG
;
Lei DONG
Author Information
- Publication Type:Journal Article
- Keywords: honokiol; pancreatic cancer; cell stemness; glycolysis; ERK
- From: Journal of Xi'an Jiaotong University(Medical Sciences) 2025;46(5):818-823
- CountryChina
- Language:Chinese
- Abstract: Objective To explore the effects of honokiol on malignant phenotypes in PANC-1 cells and its mechanisms.Methods In our experiments,MTT assay was used to detect the effects of honokiol on PANC-1 cell viability.The migration and invasion of PANC-1 cells were evaluated by wound healing assay and Transwell assay.The glycolysis level was detected by ELISA and ECAR.Western blotting was used to detect the expressions of OCT4,SOX2,Nanog,LDHA,HK2 and Raf/MEK/ERK pathway related proteins.Results Honokiol could significantly inhibit the migration and invasion abilities of PANC-1 cells(migration rate and invasion rate being 13.78％-59.45％and 12.47％-44.47％,respectively).Mechanistically,honokiol targeted Raf/MEK/ERK signaling pathway and significantly reduced the expressions of LDHA(29.60％)and HK2(31.98％),thereby reducing glycolysis and lactate production(29.42％-56.31％,compared with control group P＜0.05).This metabolic reprogramming effect ultimately inhibited the protein expressions of OCT4(33.38％),SOX2(33.07％),and Nanog(26.24％).Conclusion Honokiol treatment can inhibit the cell viability,migration and invasion abilities of PANC-1 cells.The mechanisms are related to the inhibition of glycolysis and cell stemness by regulating Raf/MEK/ERK pathway.
