Mechanism of Shanggan granules in suppressing the TLR4/NF-κB signaling pathway to alleviate the pulmonary inflammatory response in H1N1-infected mice
10.3969/j.issn.1671-7856.2025.08.006
- VernacularTitle:上感颗粒抑制TLR4/NF-κB信号通路改善H1N1感染小鼠肺部炎症反应的机制研究
- Author:
Mao LI
1
;
Zhihong GUO
;
Linjie LIU
;
Mengnan ZHANG
;
Xiuyuan LI
Author Information
1. 西南医科大学附属中医医院呼吸内科,四川泸州 646000
- Publication Type:Journal Article
- Keywords:
H1N1 influenza virus;
Shanggan granules;
inflammatory response;
oxidative stress;
TLR4/NF-κB signaling pathway
- From:
Chinese Journal of Comparative Medicine
2025;35(8):58-66
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the mechanism of Shanggan granules in suppressing pulmonary inflammation in mice infected with H1N1 influenza virus.Methods A mouse model of pulmonary influenza virus infection was established by nasal inoculation with H1N1 influenza virus.Mice were divided into a normal control group,model group,positive control group,and low-,medium-,and high-dose Shanggan granules groups.Mice were treated for 7 days and then sacrificed,and the body weight and lung wet weight were measured.Pathological changes in the lung tissues were detected by hematoxylin/eosin(HE)staining.Tumor necrosis factor-α(TNF-α),interleukin(IL)-6,IL-8,and transforming growth factor-β(TGF-β)levels in lung tissues were detected by enzyme-linked immunosorbent assay,and superoxide dismutase(SOD),glutathione peroxidase(GSH-Px),and malondialdehyde(MDA)were detected using appropriate kits.Toll-like receptor 4(TLR4)/nuclear factor-κB(NF-κB)inflammatory signaling pathways were detected by real-time polymerase chain reaction,and TANK-binding kinase 1(TBK1)/interferon regulatory factor(IRF)signaling pathway proteins were detected by Western blot.Results Both Shanggan granules and oseltamivir phosphate reduced the lung wet weight(P＜0.05,P＜0.001)in mice infected with influenza virus H1N1 compared with the model group,decreased the infiltration of inflammatory cells in lung tissue,reduced levels of the inflammatory factors TNF-α,IL-6,IL-8,and TGF-β(P＜0.05,P＜0.01,P＜0.001),decreased levels of SOD and GSH-Px in lung tissue(P＜0.05,P＜0.01),and increased MDA levels(P＜0.05,P＜0.01).Shanggan granules and oseltamivir phosphate also reduced TLR4,MyD88,and p38 mRNA levels(P＜0.05,P＜0.01)and expression of TBK1/IRF3/7/NF-κB signaling pathway proteins(P＜0.05,P＜0.01,P＜0.001).Conclusions Shanggan granules may effectively reduce lung injury,lung inflammation,and oxidative stress,via a mechanism related to the down-regulation of TLR4/NF-κB inflammatory signaling pathways.
