Study on the anti-hepatocellular carcinoma mechanism of H22 liver cancer-bearing mice of Chaiqi Yigan granule in improving intestinal mucosal permeability regulation flora
10.13699/j.cnki.1001-6821.2024.22.017
- VernacularTitle:柴芪益肝颗粒改善H22肝癌荷瘤小鼠肠道黏膜通透性调控菌群的抗肝癌作用机制研究
- Author:
Xu PANG
1
;
Xiao-bin LI
;
Tong SUN
;
Bo-wen LIU
;
Shi-ping HU
Author Information
1. 北京中医药大学东直门医院肝病科,北京 100700
- Publication Type:Journal Article
- Keywords:
Chaiqi Yigan granule;
liver cancer;
intestinal flora;
intestinal mucosal permeability
- From:
The Chinese Journal of Clinical Pharmacology
2024;40(22):3301-3305
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the therapeutic effect of Chaiqi Yigan granules on H22 liver cancer-bearing mice and its effect on intestinal flora.Methods The mouse model of liver cancer was constructed by subcutaneous inoculation of H22 cell suspension.The rats were divided into normal group,model group(H22 liver cancer-bearing model),positive control group(50 mg·kg-1 Sorafenib)and experimental-L,-H groups(12.42,49.68 g·kg-1 Chaiqi Yigan granules).Stripping the tumor,weighing the tumor.The serum marker alpha-fetal protein(AFP)of liver cancer was detected by enzyme-linked immunosorbent assay(ELISA)method.The intestinal flora was detected by 16 s rRNA.The expression of tight junction proteins Occludin and zonula occludens protein-1(ZO-1)in colonic mucosa and the changes of intestinal mucosal permeability were observed by immunohistochemical staining.Results The tumor weights in model group,experimental-L group,experimental-H group and positive control group were(1.18±0.08),(1.13±0.10),(0.84±0.13)and(0.55±0.07)g;the expression of AFP were(7.93±0.21),(7.74±0.23),(7.25±0.53)and(5.59±0.03)ng·mL-1,respectively.There were significant differences in the above indexes between the experimental-H group and the model group(P＜0.01).The Bacteroidota in normal group,model group,experimental-L group,experimental-H group and positive control group were(32.10±8.67)％,(12.72±4.31)％,(50.34±13.48)％,(28.37±6.17)％and(44.47±2.85)％;Firmicutes were(67.18±4.20)％,(82.47±4.64)％,(38.16±12.88)％,(68.39±5.83)％and(52.61±2.86)％;Lactobacillaceae were(12.20±3.25)％,(1.85±0.63)％,(9.17±2.47)％,(3.56±0.74)％and(27.83±6.77)％,respectively.There were significant differences in the above indexes between the experimental-H group and the model group(P＜0.01,P＜0.05).The results of colonic immunohistochemistry showed that the cells in the model group were loosely arranged,the intercellular proteins were reduced,and the intercellular space was increased,resulting in increased mucosal permeability.With the increase of the dose of Chaiqi Yigan granules,Occludin protein and ZO-1 protein gradually increased,making the cells arranged neatly and orderly,the intercellular space became smaller,and the permeability of the mucosa gradually returned to normal.Conclusion Chaiqi Yigan granules may play an anti-hepatocellular carcinoma role by improving intestinal mucosal permeability and regulating intestinal flora.
