Effects of Jinshui Liujun decoction on airway inflammation and IFN-γ/IL-4 imbalance in asthmatic mice
10.13699/j.cnki.1001-6821.2024.22.013
- VernacularTitle:金水六君煎对哮喘小鼠气道炎症及IFN-γ/IL-4失衡的影响研究
- Author:
Zhi-wang WANG
1
;
Jian-hong XI
;
Ke-ke LIANG
;
Ke-ting HUANG
;
Yue ZHAO
;
Ying YANG
;
Xiao-li CHENG
;
Hai-jing DUAN
;
Yuan REN
Author Information
1. 甘肃中医药大学药学院,甘肃兰州 730000;甘肃中医药大学陇药产业创新研究院,甘肃兰州 730000
- Publication Type:Journal Article
- Keywords:
Jinshui Liujun decoctions;
asthma;
airway inflammation;
interferon-γ/interleukin-4 immune imbalance
- From:
The Chinese Journal of Clinical Pharmacology
2024;40(22):3280-3284
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effects of Jinshui Liujun decoction(JLD)on airway inflammation and interferon-γ(IFN-γ)/interleukin-4(IL-4)imbalance in asthma.Methods The ovalbumin(OVA)was used to replicate the asthmatic mouse by injection and nebulization inhalation.Asthmatic mice were randomly divided into model group,positive control group(1.0 mg·kg-1 dexamethasone)and experimental-L,-M,-H groups(4.1,8.2 and 16.4 g·kg-1 JLD).The blank group and the model group were given purified water by gavage for 2 consecutive weeks.The lung function[tidal volume(TV)and respiratory rate(RR)]of mice was measured by lung function test system after administering JLD by gavage.IFN-γ,IL-4 in bronchoalveolar lavage fluid(BALF)and their genes in lung tissue were determined through enzyme-linked immunosorbent assay and quantitative real-time polymerase chain reaction respectively.Results The TV levels in blank group,model group,positive control group and experimental-L,-M,-H groups were(118.90±24.20),(87.10±13.36),(112.70±18.92),(105.20±15.24),(107.60±16.47)and(110.10±17.41)μL;RR levels were(126.60±17.57),(178.80±31.06),(133.00±19.45),(146.00±25.82),(141.30±24.78)and(137.00±22.81)bpm;IFN-γ levels were(110.95±9.26),(60.16±2.75),(98.09±8.21)、(79.58±6.49)、(88.16±7.58)and(97.26±7.23)pg·mL-1;IL-4 levels were(118.06±8.47)、(416.08±22.21)、(272.17±13.14)、(319.34±15.27)、(298.77±14.83)and(278.71±14.24)pg·mL-1;the IFN-γ mRNA levels were 2.70±0.08,1.00±0.00,2.65±0.06,1.89±0.04,2.24±0.05 and 2.58±0.06;IL-4 mRNA levels were 0.25±0.02,1.00±0.00,0.26±0.02,0.61±0.05,0.50±0.03 and 0.33±0.03,respectively.There were statistically significant differences between the above indicators of model group and blank group,and experimental-L,-M,-H groups and model group(P＜0.05,P＜0.01).Conclusion JLD has a certain antiasthmatic effect and one of mechanisms is to alleviate the IFN-γ/IL-4 immune imbalance and inhibit airway inflammation.
