Detection of the Level of Sperm Flagellar Tubulin Glycosylation in Asthenospermia and Evaluate the Clinical Value
10.3969/j.issn.1671-7414.2025.05.027
- VernacularTitle:弱精子症患者精子鞭毛微管蛋白糖基化水平检测及其临床价值研究
- Author:
Jing LI
1
;
Jifang SUN
;
Miao LIU
;
Erdaini AI
;
Zhiyong WANG
;
Ruijun LIU
;
Yan WU
;
Zhuoyun TONG
Author Information
1. 新疆维吾尔自治区妇幼保健院生殖医学中心,乌鲁木齐 830000
- Publication Type:Journal Article
- Keywords:
infertility;
asthenospermia;
tubulin glycosylation;
sperm flagella
- From:
Journal of Modern Laboratory Medicine
2025;40(5):145-148,166
- CountryChina
- Language:Chinese
-
Abstract:
Objective To examine the level of sperm flagellar tubulin glycosylation(Gly-Tubulin)in asthenospermia patients(AP)and discuss its significance.Methods According to the principle of inclusion and exclusion criteria,108 patients treated in the Department of Andrology in Xinjiang Uygur Autonomous Region Maternal and Child Health Hospital from June 2021 to May 2022 were rolled in this study.According to the results of semen examine,they were divided into group AP(n=74),and normal control(NC)group(n=34).Colleced the clinical date of both group,such as the age,the sterility period.The levels of sex hormones were detected by chemiluminescence method.The glycosylated Tubulin levels of the sperm were detected by Western blot and immunofluorescence was used for localization analysis,and the relationship between the Gly-Tubulin of sperm flagellar and asthenospermia was analyzed by Multivariate Logistic regression analysis.Results Compared to NC group,the sterility period of group AP was prolong(3.98±0.30 vs 0.78±0.08),the percent of forward sperm motility was decreased(18.55±0.97 vs 46.24±1.89)and the expression level of Gly-Tubulin in the sperm flagellar was decreased(0.64±0.11 vs 1.00±0.00),the differences with statistical significance(t=62.038,χ2=14.394,3.148,all P＜0.05).There were no significant differences in follicle-stimulating hormone(FSH),luteinizing hormone(LH),prolactin(PRL),estradiol(E2),testosterone,semen volume,sperm concentration and total sperm between the two groups(t/χ2=0.043～1.503,all P>0.05).Multivariate Logistic regression analysis showed that the percent of forward sperm motility and the sperm flagellar Gly-Tubulin expression level were independent risk factors for male asthenospermia(Wald χ2=4.406,7.492,P<0.05).Conclusion Abnormal level of Gly Tubulin in sperm flagella may be closely related to the occurrence and development of asthenospermia.
