Determination of ciprofol and propofol in human plasma by LC-MS/MS method
10.13699/j.cnki.1001-6821.2024.21.018
- VernacularTitle:用LC-MS/MS法测定人血浆中环泊酚与丙泊酚的浓度
- Author:
Ming LU
1
;
Xu-hua HUO
;
Jin-gui GAO
;
Wen-li LI
;
Wen-hao CHU
;
Zhi-qing ZHANG
Author Information
1. 河北医科大学第二医院药学部,河北石家庄 050000
- Publication Type:Journal Article
- Keywords:
ciprofol;
propofol;
liquid chromatography/mass spectrometry;
concentration of plasma
- From:
The Chinese Journal of Clinical Pharmacology
2024;40(21):3158-3162
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a method for determination of ciprofol and propofol in human plasma and apply it to detect the drug concentration of general anesthesia in patients plasma undergoing elective surgery.Methods Plasma samples were precipitated with acetonitrile,and Symmetry C18 column(4.6 mm x 150.0 mm,3.5 pm)was selected and the mobile phase consisted of acetonitrile(B)and 0.01％ammonia water(containing 5 mmol·L-1 ammonium acetate;A)with gradient elute.The internal standard was D6-ciprofol.Electrospray power supply and negative ionization of multi-reaction monitoring were used for mass spectrum conditions.The specificity,linearity and lower limit of quantitation,precision,recovery rate,and matrix effects of the method were investigated.Results The concentration of ciprofol in human plasma was linear in the range of 15-2 000 ng·mL-1;and the standard curve equation was y=1.94 × 10-3x+8.17 × 10-3.The concentration of propofol in human plasma was linear in the range of 30-4 000 ng·mL-1;and the standard curve equation was y=3.29 × 10-3x+6.90 × 10-2.The relative standard deviation values of ciprofol and propofol for intra-day and inter-day precision were less than 9％,and the absolute recoveries were both above 90％.The matrix effects of the analytes were within the range of±7％.The concentrations of ciprofol and propofol in plasma samples of 21 patients were 388.82-1 135.66 ng·mL-1 and 1 015.98-2 796.43 ng·mL-1,respectively.Conclusion The method established conformed to the standards for analyzing biological samples is convenient with high accuracy,and can simultaneously determine the concentrations of ciprofol and propofol in human plasma,which is suitable for clinical blood concentration monitoring and human pharmacokinetic study.
