Correlations between preoperative serum levels of GINS4 and PD-1 and clinicopathological characteristics and prognosis in patients with gastric cancer
10.12007/j.issn.0258-4646.2025.02.011
- VernacularTitle:胃癌患者术前血清GINS4、PD-1水平与临床病理特征及预后的相关性
- Author:
Yaoyu TANG
1
;
Like ZHANG
;
Chunyan LUO
Author Information
1. 南阳市第一人民医院检验科,河南南阳 473000
- Publication Type:Journal Article
- Keywords:
gastric cancer;
GINS complex 4;
PD-1;
clinicopathological characteristics;
prognosis
- From:
Journal of China Medical University
2025;54(2):156-160,166
- CountryChina
- Language:Chinese
-
Abstract:
Objective To analyze the expression levels of serum GINS complex 4(GINS4)and PD-1 in patients with gastric cancer before surgery and to explore the relationship between these two factors and the clinicopathological characteristics and prognosis of these patients.Methods A total of 95 patients with gastric cancer treated at the First People's Hospital of Nanyang between August 2016 and August 2018 were included in this study.The patients were followed-up for 5 years and divided into survival and death groups based on their survival at the end of the follow-up.A total of 95 healthy individuals in the same period were selected as the control group.The serum PD-1 levels before surgery were measured using an enzyme-linked immunosorbent assay(ELISA).The level of serum GINS4 mRNA before surgery was detected with real-time quantitative PCR.The relationship between serum GINS4 mRNA and PD-1 levels prior to surgery and the 5-year survival rate in the patients with gastric cancer were analyzed using the Kaplan-Meier method.Cox regression was used to analyze the factors affecting gastric cancer prognosis.Results The serum levels of GINS4 mRNA and PD-1 were significantly higher in the patients with gastric cancer than in the healty controls(P＜0.05).The serum levels of GINS4 mRNA and PD-1 in patients with poorly differentiated tissues in TNM stages Ⅲ and Ⅳ,and lymph node metastasis were significantly higher than those in patients with moderately/highly differentiated tissues,in TNM stage Ⅱ,and no lymph node metastasis(P＜0.05).The serum levels of GINS4 mRNA and PD-1,proportio of patients with TNM stages Ⅲ and Ⅳ,and the proportion of patients with lymph node metastasis in the death group were significantly higher than those in the survival group(P＜0.05).According to the results of Kaplan-Meier analysis,the 5-year survival rate of patients with gastric cancer with high serum expression levels of GINS4 mRNA and PD-1 was lower than that of patients with low expression levels of GINS4 mRNA and PD-1(P＜0.05).GINS4 mRNA and PD-1 levels were independent risk factors for death in patients with gastric cancer(P＜0.05).Conclusion The expression levels of serum GINS4 mRNA and PD-1 in patients with gastric cancer were higher than those of the controls,which were related to tissue differentiation,TNM staging,lymph node metastasis,and prognosis.
