circ_0008043 promotes glycolysis of hepatocellular carcinoma and the development of hepatocellular carcinoma through miR-198/SLC2A1 axis
10.11904/j.issn.1002-3070.2024.06.007
- VernacularTitle:circ_0008043通过miR-198/SLC2A1轴促进肝癌糖酵解及肝癌发展
- Author:
Kangjun ZHANG
1
;
Taishi FANG
1
;
Fulan CEN
1
;
Xu YAN
1
;
Qijun CHEN
1
;
Nan MA
1
Author Information
1. 深圳市第三人民医院肝脏外科(肝移植)(南方科技大学第二附属医院)(深圳 518000)
- Publication Type:Journal Article
- Keywords:
Hepatocellular carcinoma;
Glycolysis;
circ_0008043;
miR-198;
SLC2A1
- From:
Practical Oncology Journal
2024;38(6):388-401
- CountryChina
- Language:Chinese
-
Abstract:
Objective The aim of this study was to investigate the mechanism of circ_0008043 regulating glycolysis of hepa-tocellular carcinoma(HCC)cells.Methods The expression of circ_0008043,miR-198 and SLC2A1 in clinical samples and HCC cell lines was detected by qPCR,and the targeted binding of circ_0008043 to miR-198 and miR-198 to SLC2A1 was verified by dual luciferase reporting assay.HCC cells were transfected with sh-circ_0008043/sh-NC,miR-198 inhibitor/miR-198 inhibitor-NC,miR-198 mimic/mimic NC,or pcDNA3.1-SLC2A1/pcDNA3.1-NC to detected the effects of circ_0008043,miR-198 and SLC2A1 on the glycolysis of HCC cells.The effect of circ_0008043 on tumor growth in HCC was verified by HCC cell xenografts through a nude mouse model.Results circ_0008043 was highly expressed in both HCC tissues and cell lines(P<0.01).Silencing circ_0008043 could inhibit the glycolysis of HCC cells.The expression of miR-198 was decreased in HCC tissues and cell lines(P<0.001)and neg-atively correlated with the expression of circ_0008043(r=-0.550,P<0.001).The inhibition of glycolysis caused by silencing circ_0008043 was partially restored by inhibiting miR-198 expression.SLC2A1 was highly expressed in HCC tissues and cell lines(P<0.001),and overexpression of SLC2A1 reversed the inhibitory effect of miR-198 on glycolysis in HCC cells.The nude mouse experi-ment showed that silencing circ_0008043 could inhibit the growth of HCC cell xenografts(P<0.001).Conclusion circ_0008043 promotes HCC cell glycolysis and growth through the miR-198/SLC2A1 axis.
