Effect of Puerariae lobatae Radix and Salviae miltiorrhizae Radix et Rhi-zoma on adipokine homeostasis in mice with diabetes-related atheroscle-rosis
10.3969/j.issn.1000-4718.2024.12.006
- VernacularTitle:葛根-丹参对糖尿病相关动脉粥样硬化小鼠脂肪因子稳态的影响
- Author:
Yu QIAO
1
;
Xiaolei WANG
1
;
Changjiang WU
1
;
Jinxu SUN
1
;
Wenhui LI
1
;
Xiangyu GUO
1
Author Information
1. 北京中医药大学东方医院,北京 100078
- Publication Type:Journal Article
- Keywords:
Puerariae lobatae Radix;
Salviae miltiorrhizae Radix et Rhizoma;
diabetes mellitus;
atheroscle-rosis;
adipokine
- From:
Chinese Journal of Pathophysiology
2024;40(12):2238-2244
- CountryChina
- Language:Chinese
-
Abstract:
AIM:This study aims to investigate the mechanisms by which Gegen(Puerariae lobatae Radix)and Danshen(Salviae miltiorrhizae Radix et Rhizoma)intervene in diabetes mellitus(DM)-related atherosclerosis(AS)through the regulation of adipokine homeostasis.METHODS:A mouse model of DM-related AS was established in male apolipoprotein E gene knockout(ApoE-/-)mice by high-fat diet feeding and intraperitoneal injection of streptozotocin.The mice were randomly divided into model group,low-dose Gegen-Danshen group,high-dose Gegen-Danshen group and posi-tive control drug metformin group,while male C57BL/J mice served as blank control group,with 6 mice in each group.Se-rum lipid levels of the mice were assessed.Oil red O staining was employed to observe pathological changes in the abdomi-nal aorta,while HE staining was used to examine the morphological changes of adipose tissues surrounding abdominal or-gans.The protein expression levels of adipokines,including adiponectin,apelin,chemerin and resistin,in adipose tis-sues were measured using RT-qPCR and Western blot.RESULTS:The administration of Gegen and Danshen resulted in a significant increase in the protein expression levels of adiponectin and apelin in adipose tissues(P＜0.01).Conversely,the levels of chemerin and resistin were significantly decreased(P＜0.01),and there was a notable reduction in low-densi-ty lipoprotein cholesterol(P＜0.01).Results of oil red O staining revealed that the aortic AS plaques of the mice in model group were numerous,large and scattered,whereas those in high-dose Gegen-Danshen group were fewer and smaller.CONCLUSION:Gegen and Danshen play a protective role in preventing DM-related AS in mice by enhancing the expres-sion of adiponectin and apelin but inhibiting the expression of chemerin and resistin.
