Molecular ITS2 gene identification of Aedes albopictus in the tropical environment of Hainan Island
10.3969/j.issn.1002-2694.2024.00.161
- VernacularTitle:基于ITS2基因对海南岛热带环境白纹伊蚊的分子鉴定
- Author:
Yi-jia XU
1
;
Xing-zhi FENG
;
Yu-ni WANG
;
Ming-yu LI
;
Qian-feng XIA
;
Ya-jun LU
Author Information
1. 国家卫生健康委员会热带病防治重点实验室,海南医科大学热带医学院,海口 571199
- Publication Type:Journal Article
- Keywords:
Hainan Island;
Aedes albopictus;
ITS2 gene;
genetic diversity;
phylogenetic relationship;
molecular identifica-tion
- From:
Chinese Journal of Zoonoses
2024;40(11):1003-1008
- CountryChina
- Language:Chinese
-
Abstract:
Through molecular identification of the ITS2 gene of Aedes albopictus in Hainan Island,we analyzed the genetic diversity,haplotype characteristics,and phylogenetic relationships,to characterize the genetic structure of the local Aedes al-bopictus population.Aedes albopictus samples were collected from 18 administrative cities and counties in Hainan Island.Ge-nomic DNA was extracted,and the 1TS2 gene was amplified.The base composition was analyzed in MEGA 11 software.The genetic distances were calculated,and the phylogenetic relationships were constructed.DnaSP6 and Pop Art 1.7 software were used to calculate the number of haplotypes,draw a haplotype topology network diagram,and analyze haplotype diversity.A to-tal of 360 ITS2 genes were obtained from Aedes albopictus in 18 cities and counties in Hainan Island,with sequence lengths ranging from 536 to 586 bp;GC content ranging from 51.29％to 55.31％;average intraspecies genetic distances ranging from 0.015 4;a total of 251 haplotypes and a haplotype diversity index of 0.987 0,indicating rich genetic diversity and intricate kin-ship relationships,the phylogenetic relationship between Aedes albopictus in Hainan Island and in Honduras was the closest.The existence of extensive gene exchange and rich genetic diversity within the Aedes albopictus population in Hainan Island provided sufficient genetic resources for the evolution and adaptation of the population.
