Correlation of long non-coding ribonucleic acid antisense non-coding RNA in the INK4 locus and long non-coding ribonucleic acid maternally expressed gene 3 levels with neurological impairment in patients with acute ischemic stroke
10.3760/cma.j.cn115455-20250423-00364
- VernacularTitle:急性缺血性脑卒中患者细胞周期激酶抑制因子4基因座中反义非编码RNA及其母体表达基因3水平与神经功能损伤的相关性研究
- Author:
Wei YAO
1
;
Na KONG
1
;
Jin LI
1
;
Xiao ZHANG
1
;
Hui SUN
1
;
Yan LU
1
;
Tongdan TANG
1
Author Information
1. 大连理工大学附属中心医院急诊内科病房，大连 116033
- Publication Type:Journal Article
- Keywords:
Thromboembolism;
Ischemic stroke;
Acute phase;
Long non-coding RNAs;
Inflammatory factors;
Dynamic pattern of change
- From:
Chinese Journal of Postgraduates of Medicine
2025;48(11):975-980
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the dynamic changes of serum long non-coding ribonucleic acid antisense non-coding RNA in the INK4 locus (LncRNA ANRIL), long non-coding ribonucleic acid maternally expressed gene 3 (LncRNA MEG3) and inflammatory factors [interferon-γ (IFN-γ), interleukin-6 (IL-6), interleukin-18 (IL-18) and interleukin-1β (IL-1β)] in patients with acute ischemic stroke and their correlation with neurological impairment.Methods:This prospective study included fifty-two patients with acute ischemic stroke admitted to the Central Hospital of Dalian University of Technology were included, and the neurological impairments of the patients were scored using the National Institutes of Health stroke scale (NIHSS) on the first day of the onset of the disease, and serum LncRNA ANRIL, LncRNA MEG3 and inflammatory factors (IFN-γ, IL-6, IL-18 and IL-1β) were detected using enzyme-linked immunosorbent assay (ELISA) at the first, second and seventh day of the onset of the disease. The correlation between serum LncRNA ANRIL, LncRNA MEG3 and inflammatory factor levels and neurological impairment at each time point was analyzed by Spearman correlation analysis.Results:Among 52 patients, mild group had 22 cases (NIHSS score <5 scores), and moderate-to-severe group had 30 cases (NIHSS score ≥5 scores). In acute ischemic stroke patients with moderate-to-severe group, the expression level of LncRNA MEG3 was higher than in the mild group, but the intergroup difference did not reach statistical significance ( P>0.05). LncRNA ANRIL, LncRNA MEG3, IFN-γ, IL-18 and IL-1β peaked at the same time point in acute ischemic stroke patients, all within second day of the onset of the disease. Spearman correlation analysis showed that the serum LncRNA MEG3 level on the first day of the onset of the disease was significantly positively correlated with NIHSS score ( P = 0.006), and serum LncRNA ANRIL level on seventh day was significantly positively correlated with NIHSS score ( P = 0.049). Conclusions:Serum LncRNA ANRIL, LncRNA MEG3 and inflammatory factors (IL-18, IL-1β and IFN-γ) are highly expressed in patients with acute ischemic stroke, peaking on the second day of onset. LncRNA MEG3 levels on the first day of onset and LncRNA ANRIL levels on the seventh day of onset are helpful in determining the degree of neurological impairment in patients with acute ischemic stroke.
