Effect of SCD1 inhibition on fatty acid metabolism pathway and the biological behavior of cervical cancer cells
10.12007/j.issn.0258-4646.2025.02.004
- VernacularTitle:抑制SCD1活性对调节脂肪酸代谢途径和抑制宫颈癌细胞肿瘤生物学行为的作用
- Author:
Junqin YU
1
;
Dan LU
;
Lanling FAN
;
Yongguo YANG
;
Hua TIAN
Author Information
1. 扬州大学附属江都人民医院妇产科,江苏扬州 225200
- Publication Type:Journal Article
- Keywords:
cervical cancer;
HeLa cells;
stearoyl-CoA desaturase 1;
fatty acid metabolism;
biological behavior
- From:
Journal of China Medical University
2025;54(2):115-120
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of inhibiting stearoyl-CoA desaturase 1(SCD1)activity on the biological behavior of cervical cancer cells and elucidate the molecular mechanisms related to fatty acid metabolism.Methods Cancer tissues were collected from 48 patients with cervical cancer,whereas normal cervical tissues were obtained from 48 patients with uterine fibroids.Immunohisto-chemical SP staining and real-time quantitative PCR were performed to assess differences in SCD1 expression.The MTS assay was used to determine cell viability,and EdU staining was conducted to evaluate cell proliferation.Cell migration and invasion abilities were analyzed using in vitro scratch assay and Transwell chamber assay.Immunofluorescence staining was performed to detect E-cadherin and vimentin expression.Real-time quantitative PCR was used to measure the mRNA expression levels of SREBP1,ACLY,ACC,and FAS.Nile red fluorescence staining was applied to observe intracellular lipid droplet content.Results The positive expression rate of SCD1 and the relative expression level of SCD1 mRNA were significantly higher in cervical cancer tissues than in normal cervical tissues(P＜0.05).Treatment of HeLa cells with varying concentrations of MF-438 led to significant reductions in cell viability,EdU-positive cell rate,scratch closure rate,and the number of migrating and invading cells.Additionally,the fluorescence intensity of E-cadherin increased,whereas that of vimentin decreased.The relative expression levels of SREBP1,A CL Y,ACC,and FAS mRNA were downregulated,and intracellular lipid droplet content decreased in a concentration-dependent manner(P＜0.05).Conclusion Inhibition of SCD1 activity significantly reduced lipid metabolism in HeLa cells and suppressed malignant biological behaviors,including proliferation,migration,invasion,and epithelial-mesenchymal transition.
