Astragaloside Ⅳ inhibits LPS-induced RAW 264.7 macrophage polarization and regulates their migration via cGAS/STING/NF-κB pathway
- VernacularTitle:黄芪甲苷通过cGAS/STING/NF-κB通路抑制LPS诱导的RAW 264.7巨噬细胞极化调控其迁移
- Author:
Chang-chao YANG
1
;
Guo-ting LI
;
Lin LIU
;
Zi-xian ZHAO
;
Wei-kang LI
;
Qing-xin SUN
;
Yu-ying ZHAO
;
Jing-shan ZHAO
Author Information
- Publication Type:Journal Article
- Keywords: astragaloside Ⅳ; macrophage polariza-tion; macrophage migration; molecular docking; li-popolysaccharide; cGAS/STING/NF-κB
- From: Chinese Pharmacological Bulletin 2025;41(7):1290-1297
- CountryChina
- Language:Chinese
- Abstract: Aim To explore the effect of astragalosideⅣ(AS-Ⅳ)on lipopolysaccharide(LPS)-induced po-larization and migration of RAW 264.7 macrophages and the underlying mechanism.Methods 1 mg·L-1 LPS was used to construct cell migration model.Scratch assay was utilized to determine cell migration rate.Immunofluorescence staining was utilized to de-tect the expression and location of F4/80,iNOS and Arg-1.CCK-8 assay was used to determine the viabili-ty of RAW 264.7 cells.Griess assay was used to measure NO content.Molecular docking was used to analyze the interaction between AS-Ⅳ and the core tar-gets such as cGAS and STING protein.Western blot was employed to detect the expression of iNOS,Arg-1,cGAS,STING,NF-κB p65 and p-NF-κB p65 protein.Results AS-Ⅳ significantly inhibited the migration and M1 polarization of RAW 264.7 cells induced by LPS.Moreover,AS-Ⅳ could interact with cGAS and STING protein,especially cGAS.Further Western blot assay showed that AS-Ⅳ significantly downregulated the expression of iNOS,cGAS,STING and p-NF-κB p65 protein.Conclusions AS-Ⅳ could promote mac-rophage M1 to M2 polarization,thereby inhibited mac-rophage migration through restraining the cGAS/STING/NF-κB signaling pathway,which provides a new therapeutic target for AS-Ⅳ to improve the early inflammatory response of AS.
