Effect of puerarin on myocardial injury in diabetic mice via NLRP3-caspase-1-GSDMD signaling pathway
- VernacularTitle:葛根素通过NLRP3/caspase-1/GSDMD信号通路对糖尿病小鼠心肌损伤的影响
- Author:
Qiu-yan CHEN
1
;
Lu WANG
;
Ren-bin HUANG
;
Xiao-hui XU
Author Information
- Publication Type:Journal Article
- Keywords: puerarin; diabetic; myocardial injury; py-roptosis; inflammation; NLRP3/caspase-1/GSDMD
- From: Chinese Pharmacological Bulletin 2025;41(7):1259-1264
- CountryChina
- Language:Chinese
- Abstract: Aim To study the protective effect of puer-arin(PR)on myocardial injury in diabetic mice and to explore the related mechanism.Methods Eight male C57BL/6J mice were randomly selected as the control group,and the remaining mice were randomly injected with streptozotocin(STZ)to construct a diabetes mod-el,and the mice that were successfully modeled were randomly divided into the model group,PR high,medi-um and low dose groups,pyroptosis inhibitor(NSA)group and pyroptosis inducer(Nigericin)group,which were administered continuously for 12 weeks.After the last dose,the serum and heart tissues of mice were taken to observe the changes of myocardium,the con-tents of lactate dehydrogenase(LDH)and creatine ki-nase isoenzyme(CK-MB)in the serum were detected,and the protein and mRNA expressions of pyroptosis-related factors(NLRP3,caspase-1,GSDMD,IL-18,IL-1β)in myocardial tissues of mice were detected.Re-sults Compared with the control group,the model group had swollen and twisted myocardial tissue,myo-cardial arrangement disorder,increased collagen fibers,increased content of LDH and CK-MB in serum,and significantly increased the expression of pyroxosis relat-ed factors in myocardial tissue(P＜0.05 or P＜0.01).Compared with the model group,the pathologi-cal changes of myocardial tissue in in the PR and NSA groups were significantly improved,the content of ser-um LDH and CK-MB was significantly reduced,and the expression of pyroxosis related factors in myocardial tis-sue was significantly inhibited(P＜0.05 or P＜0.01),while there was no significant change in the Nigericin group.Conclusions PR has a protective effect on myocardial injury in diabetic mice,and its mechanism may be related to the inhibition of the NL-RP3/caspase-1/GSDMD pyrosis signaling pathway.
