Effect of total flavones of Coreopsis tinctoria Nutt on vascular dementia by inhibiting miR-93-mediated TLR4 signaling pathway and its mechanism
- VernacularTitle:两色金鸡菊总黄酮抑制miR-93调控TLR4信号通路改善血管性痴呆的作用及机制
- Author:
Meng-ying HU
1
;
Dong-mei YANG
;
Yi-zhong ZHU
;
Qin-lan LIANG
;
Houwati NUERBAHETI
;
Xiao-jun YANG
;
Hasimu HAMULATI
Author Information
- Publication Type:Journal Article
- Keywords: total flavones of Coreopsis tinctoria Nutt; vascular dementia; miR-93; TLR4 signaling pathway; cognitive impairment; inflammatory response
- From: Chinese Pharmacological Bulletin 2025;41(7):1237-1244
- CountryChina
- Language:Chinese
- Abstract: Aim To investigate the effect of total fla-vones of Coreopsis tinctoria Nutt(CF)on cognitive im-pairment in vascular dementia(VD).Methods The VD rat models were established by modified bilateral common carotid arteries ligation method.SD rats were divided into the sham operation group,model group,positive control group(nicergoline),and low,medium,and high dose CF groups.After eight weeks of admin-istration,the short term memory and spatial learning and memory abilities were evaluated by the platform jumping test,dark avoidance test and Morris water maze test.The pathological changes of the hippocam-pal tissues were inspected by HE and Nissl staining.The contents of TNF-α and IL-1β in the hippocampal were examined by ELISA.The protein expression lev-els of TLR4,MyD88,NF-κB p65,and p-NF-κB p65 in the hippocampal were detected by Western blot.The mRNA expression levels of miR-93,TLR4,MyD88,and NF-κB p65 in the hippocampal were determined by qRT-PCR.Results CF obviously improved the short term memory and spatial learning and memory abilities of VD rats,and alleviated the pathological damage of the hippocampus.CF also obviously decreased the lev-els of TNF-α and IL-1β,declined the protein expres-sion levels of TLR4,MyD88,and p-NF-κB p65,and re-duced the miR-93,TLR4,and MyD88 mRNA expres-sion in the hippocampus.Conclusion CF has a nota-ble protective effect on the neuroinflammation and cog-nitive impairments in VD rats by inhibiting the miR-93-mediated TLR4 signaling pathway.
