Isolation,identification,and analysis of drug resistance and virulence genes in Escherichia coli isolated from artificially bred sika deer
10.3969/j.issn.1002-2694.2025.00.072
- VernacularTitle:人工繁育梅花鹿源大肠埃希菌分离鉴定及耐药性与毒力基因分析
- Author:
Cheng-yang ZHANG
1
;
Xue JI
;
Bo-wen JIANG
;
Bing LIANG
;
Rong-lei HUANG
;
Chong-tao DU
;
Yang SUN
Author Information
1. 吉林大学动物医学学院,长春 130062;中国农业科学院长春兽医研究所,长春 130122;吉林省人兽共患病预防与控制重点实验室,长春 130122
- Publication Type:Journal Article
- Keywords:
sika deer;
Escherichia coli;
drug resistance;
virulence gene;
ERIC-PCR
- From:
Chinese Journal of Zoonoses
2025;41(5):522-528
- CountryChina
- Language:Chinese
-
Abstract:
To understand the background of Escherichia coli(E.coli)carried by artificially bred sika deer and the biological characteristics of the isolated strains,such as drug resistance and pathogenicity,in April 2024,we collected 184 fresh deer fecal samples from four deer farms in Luxiang Township,Shuangyang District,Changchun City,Jilin Province,for isolation and cultivation of E.coli.The isolates were tested for drug resistance and biochemical identification with a BD PhoenixTM-100 Automated Microbiology System.The virulence genes were detected with PCR,and the strains were molecularly typed with ERIC-PCR.A total of 165 E.coli strains were isolated from 184 samples of deer feces,with an isolation rate of 89.67%.Twenty strains had a drug resistance phenotype,and the drug resistance rate was 12.12%;these strains included 15 strains of multi-drug resistant bacteria and 11 strains of ESBL-producing bacteria.Virulence gene detection indicated that the sika deer isolates carried multiple diarrhea-associated virulence genes,such as EAST-1(12.12%),eae(1.21%),stx1(7.88%),stx2(7.27%),and STa(1.82%).ERIC-PCR demonstrated that the isolates showed high polymorphism.The ESBL-producing E.coli carried by sika deer are likely to spread drug resistance in the community and livestock population.Some isolates carried multiple diarrhea-associated virulence genes,thus posing a human transmission risk.Therefore,monitoring of drug resistance and virulence genes must be strengthened,and antibiotics must be used reasonably during the breeding process to avoid excessive use and misuse.
