Whole genome sequencing and analysis of multidrug resistant ST314 Salmonella Kentucky from a broiler slaughterhouse
10.3969/j.issn.1002-2694.2025.00.074
- VernacularTitle:肉鸡屠宰环节多重耐药ST314肯塔基沙门菌的全基因组序列分析
- Author:
Jia-rui LI
1
;
Rui-yuan SUN
;
Pei-jie HE
;
Hao-tian LIU
;
Ru-yi KUANG
;
Jing XIA
;
Min CUI
;
Yong HUANG
;
Li-kou ZOU
;
Xin-feng HAN
Author Information
1. 四川农业大学动物医学院,成都 611130
- Publication Type:Journal Article
- Keywords:
Salmonella Kentucky;
multidrug resistant;
extended-spectrum β-lactamase;
whole-genome sequencing
- From:
Chinese Journal of Zoonoses
2025;41(5):537-543
- CountryChina
- Language:Chinese
-
Abstract:
This study investigated the potential pathogenicity and genetic characteristics of ST314 Salmonella Kentucky(S.Ken-tucky)isolates from a broiler slaughterhouse.Antimicrobial susceptibility testing and whole-genome sequencing(WGS)were used to determine antimicrobial resistance,virulence factors,and the presence of antimicrobial resistance genes(ARGs)and mobile genetic elements(MGEs)among the isolates.The three multidrug resistant(MDR)isolates exhibited high resistance to multiple antimicrobial agents.The F4-2S strain exhibited resistance to 14 drugs across seven categories,whereas the F4T strain showed resistance to 13 drugs in the same number of categories.In contrast,the Y23 strain was resistant to nine drugs in six categories.Notably,F4-2S dem-onstrated high homology with F4T:both possessed 13 ARGs distributed across nine categories,in addition to a wide range of virulence factors,including secretion systems and effector proteins.The presence of IncR and IncX1 plasmids significantly enhanced both the antimicrobial resistance and pathogenicity of the isolates.The genome map of Y23 revealed a chromosome alongside two plasmids.The chromosome containedonly one resistance gene but several virulence factors,including the type III secretion system(T3SS),which is crucial for bacterial invasion.The plasmid pY23-1 contained eight types of 19 ARGs.Comparative analysis indicated that pY23-1 ex-hibited high homology with pZ1323SSL0055 and pSAL-045,all of which contained multiple ARGs,thus suggesting critical roles of these genes in the evolution of bacterial resistance.In conclusion,ST314 S.Kentucky demonstrated a complex mechanism of resis-tance coupled with significant pathogenic potential.The ARGs and MGEs in the plasmid contributed to the emergence and dissemina-tion of antimicrobial resistance.The multiple virulence factors present in the chromosome may be key factors driving the increasing virulence of ST314 S.Kentucky.
