Effect of lncRNA FOXCUT-mediated regulation of FOXC1 expression on prolifer-ation and invasion of non-small cell lung carcinoma cells
10.13315/j.cnki.cjcep.2025.06.009
- VernacularTitle:LncRNA FOXCUT调控FOXC1表达对非小细胞肺癌细胞增殖和侵袭的影响
- Author:
Zhen QIAN
1
;
Haitao ZHANG
;
Guoqiang FU
;
Jiajia DONG
Author Information
1. 空军军医大学唐都医院急诊科,西安 710032
- Publication Type:Journal Article
- Keywords:
lung neoplasms;
non-small cell lung carcinoma;
long non-coding RNA;
FOXCUT;
FOXC1
- From:
Chinese Journal of Clinical and Experimental Pathology
2025;41(6):751-758,764
- CountryChina
- Language:Chinese
-
Abstract:
Purpose To explore the role and mechanism by which the forkhead box C1(FOXC1)promoter up-stream transcript(FOXCUT)regulates proliferation and invasion of non-small cell lung carcinoma(NSCLC)cells.Methods Bioinformatic analysis and RT-qPCR were used to quantify FOXCUT expression in NSCLC tissues.After FOXCUT knockdown in NSCLC cell lines,cell proliferation was examined using CCK-8 and EdU assays,and invasion was evaluated by Transwell assay.The expression of E-cadherin,vimentin,N-cadherin,and FOXC1 was detected by Western blot.FOXCUT-silenced H460 cells were constructed using lentiviruses and subcutaneously injected into nude mice to observe tumor growth.To rescue FOXC1 expression,an FOXC1 expression plasmid was transfected into FOX-CUT-knockdown cells.LncBook 2.0,ENCORI,and TargetScan databases were queried to predict miRNAs that inter-act with FOXCUT and FOXC1.Results FOXCUT expression was significantly higher in NSCLC tissues than in normal lung tissues(normal:0.24±0.22 vs NSCLC:0.68±0.76,t=5.94,P＜0.001),and patients with high FOXCUT expression had a poorer prognosis(P＜0.01).FOXCUT interference markedly repressed NSCLC cells' proliferation and invasion(P＜0.01).FOXCUT knockdown significantly upregulated E-cadherin and downregulated vimentin and N-cadherin(P＜0.01).In vivo,FOXCUT-silenced cells formed significantly smaller tumors in nude mice(P＜0.01).FOXCUT knockdown markedly reduced FOXC1 expression(P＜0.01).Overexpression of FOXC1 in FOX-CUT-depleted cells rescued cell proliferation(P＜0.01).Bioinformatic analysis identified 8 miRNAs potentially co-regulated by FOXCUT and FOXC1.Conclusion Knockdown of FOXCUT restrains NSCLC cell proliferation and inva-sion,possibly through suppression of FOXC1 expression.
