Correlation between inflammatory factor levels in gingival crevicular fluid and pain degree, stress response and masticatory function in patients with combined periodontal and pulpal lesions
10.3760/cma.j.cn431274-20240515-00806
- VernacularTitle:牙周牙髓联合病变患者龈沟液炎症因子水平与疼痛程度、应激反应和咀嚼功能的相关性
- Author:
Leilei SUN
1
;
Jiawei GU
;
Guiyun REN
Author Information
1. 延安市人民医院口腔科，延安 716000
- Publication Type:Journal Article
- Keywords:
Periodontal diseases;
Dental pulp diseases;
Inflammatory factor;
Pain;
Mastication
- From:
Journal of Chinese Physician
2025;27(6):865-869
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the correlation between inflammatory factor levels in gingival crevicular fluid and pain degree, stress response and masticatory function in patients with combined periodontal and pulpal lesions.Methods:A retrospective selection of 92 patients with combined periodontal and pulpal lesions admitted to Yan ′an People′s Hospital from May 2022 to May 2023 was divided into mild group (60 cases) and moderate-severe group (32 cases) according to the degree of combined periodontal and pulpal lesions. Periodontal clinical indicators were compared between the two groups; enzyme-linked immunosorbent assay (ELISA) was used to detect inflammatory factors in gingival crevicular fluid and serum cortisol (Cor) and adrenocorticotropic hormone (ACTH) levels; Visual Analogue Scale (VAS) was used to evaluate pain degree; and weighing method was used to evaluate masticatory efficiency. Pearson correlation analysis was used to evaluate the correlation between inflammatory factor levels in gingival crevicular fluid and pain degree, stress response and masticatory function.Results:The probing depth (PD), attachment loss (AL), Plaque Index (PLI) and Bleeding Index (BI) in the moderate-severe group [(5.35±0.40)mm, (5.98±0.59)mm, 1.22±0.42, 1.28±0.43] were all higher than those in the mild group [(4.23±0.36)mm, (2.53±0.29)mm, 1.05±0.34, 0.80±0.40] (all P<0.05). The levels of interleukin-1β (IL-1β), interleukin-17 (IL-17) and high-sensitivity C-reactive protein (hs-CRP) in gingival crevicular fluid in the moderate-severe group [(41.87±11.78)μg/L, (606.32±59.33)pg/ml, (58.64±10.63)mg/L] were higher than those in the mild group [(30.35±10.53)μg/L, (478.63±43.18)pg/ml, (43.15±9.85)mg/L], and the level of transforming growth factor-β (TGF-β) [(53.98±6.98)μg/L] was lower than that in the mild group [(121.67±12.87)μg/L] ( P<0.05). The serum Cor and ACTH levels in the moderate-severe group [(165.68±34.64)μg/L, (25.97±6.37)ng/L] were higher than those in the mild group [(148.73±29.65)μg/L, (17.97±5.34)ng/L] (all P<0.05). The VAS score in the moderate-severe group [(5.03±1.20)points] was higher than that in the mild group [(3.57±1.01)points] ( P<0.05). The bite force and masticatory efficiency in the moderate-severe group [(95.32±23.73)lbs, (65.97±13.34)%] were lower than those in the mild group [(113.75±26.46)lbs, (75.89±16.86)%], and the gingival index (1.41±0.50) was higher than that in the mild group (0.85±0.36) (all P<0.05). Correlation analysis showed that IL-1β was positively correlated with disease degree, periodontal indexes, IL-17 and inflammatory factors, and negatively correlated with TGF-β; IL-17 was positively correlated with disease degree and multiple indexes, and negatively correlated with TGF-β, bite force and masticatory efficiency; TGF-β was positively correlated with bite force and masticatory efficiency, and negatively correlated with disease degree and multiple indexes. Conclusions:Inflammatory factors in gingival crevicular fluid of patients with combined periodontal and pulpal lesions are closely related to pain and stress response, and affect masticatory function. In diagnosis and treatment, inflammatory status should be comprehensively considered to provide a basis for personalized treatment.
