Effects of sleep deprivation on lactic acid levels and cognitive function in mice
10.3760/cma.j.cn431274-20240708-01053
- VernacularTitle:睡眠剥夺对小鼠体内乳酸水平及认知功能的影响
- Author:
Yingjian ZHANG
1
;
Tong PAN
;
Zhenlong LIU
;
Kai YUAN
Author Information
1. 北京大学第六医院，北京大学精神卫生研究所，北京 100191
- Publication Type:Journal Article
- Keywords:
Sleep deprivation;
Lactic acid;
Cognition
- From:
Journal of Chinese Physician
2025;27(6):837-840
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the potential effects of sleep deprivation on lactic acid metabolism and cognitive function in mice.Methods:Twelve SPF-grade BALB/c mice were randomly divided into a normal control group and a sleep deprivation group using a random number table, with 6 mice in each group. The horizontal platform method was used to establish a mouse sleep deprivation model. The Morris water maze was employed to assess cognitive ability, including initial quadrant residence time, platform crossing times, escape latency, and total swimming distance. Serum lactic acid levels and inflammatory factors such as interleukin (IL)-1β, IL-6, and tumor necrosis factor (TNF)-α were also detected.Results:There was no significant difference in body weight between the two groups before and after sleep deprivation (all P>0.05). Compared with the control group, the sleep deprivation group showed prolonged residence time in the initial quadrant, significantly shorter residence time in the target quadrant, and fewer platform crossings (all P<0.05). Escape latency and total swimming distance in the sleep deprivation group were significantly longer than those in the control group (all P<0.01). Serum lactic acid levels and inflammatory factors IL-6, IL-1β, and TNF-α in the sleep deprivation group were significantly higher than those in the control group (all P<0.05). Conclusions:Sleep deprivation impairs cognitive ability in mice, accompanied by significant increases in lactic acid and inflammatory factors.
