Role of EBP50 in renal tubular epithelial-mesenchymal transition in diabetic nephropathy
10.3760/cma.j.cn431274-20240505-00743
- VernacularTitle:EBP50在糖尿病肾病肾小管上皮-间质转化中的作用
- Author:
Xiaofei XIU
1
;
Xiaomei WANG
;
Tao ZHANG
;
Xuelan XIAO
;
Hongjing SUN
;
Zinan GUO
;
Feng GAO
Author Information
1. 河北医科大学第三医院病理科，石家庄 050051
- Publication Type:Journal Article
- Keywords:
Diabetic nephropathies;
Ezrin-radixin-moesin-binding phosphoprotein 50;
Epithelial-mesenchymal transition
- From:
Journal of Chinese Physician
2025;27(5):678-681
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the role of ezrin-radixin-moesin-binding phosphoprotein 50 (EBP50) in renal tubular epithelial-mesenchymal transition (EMT) in diabetic nephropathy (DN).Methods:Renal tissue specimens from 24 DN patients (DN group) confirmed by medical history, clinical laboratory tests, and pathological diagnosis at the Third Hospital of Hebei Medical University and 15 adjacent normal renal tissues (control group) from 2018 to 2023 were collected. Immunohistochemical staining was performed to detect the expression of EBP50, E-cadherin, and Vimentin proteins. Additionally, 10 healthy volunteers were enrolled as a normal group, and fasting blood glucose, serum creatinine, blood urea nitrogen, and 24 h urinary protein levels were compared between DN patients and the normal group.Results:Fasting blood glucose, serum creatinine, blood urea nitrogen, and 24 h urinary protein levels were significantly higher in the DN group than in the normal group (all P<0.01). Periodic acid-schiff (PAS) staining revealed significant thickening of the glomerular basement membrane, increased extracellular matrix, vacuolar degeneration of renal tubules, and elevated extracellular matrix in the renal interstitium in the DN group. Immunohistochemistry showed higher expression of EBP50 and E-cadherin proteins in the control group than in the DN group (all P<0.01), while Vimentin expression was lower in the control group ( P<0.01). Conclusions:EBP50 is involved in the EMT process of renal tubules in DN and is associated with tubular injury.
