Optimization Study of Rat Models for Sequelae of Pelvic Inflammatory Disease
10.13241/j.cnki.pmb.2025.12.001
- VernacularTitle:盆腔炎性疾病后遗症大鼠模型优化及机制探讨
- Author:
Zhen LIU
1
;
Wei-ling WANG
;
Yun-cheng MA
;
Yu-xi WANG
;
Yuan TIAN
;
Qian LI
;
Xiao-zhu WANG
;
Xiao-yao LIU
;
Mei JIANG
;
Wen-hui XU
;
Jian GAO
;
Ting WANG
Author Information
1. 北京中医药大学中药学院 北京 100102
- Publication Type:Journal Article
- Keywords:
Sequelae of pelvic inflammatory disease;
SPID;
Rat models;
Bacterial infections
- From:
Progress in Modern Biomedicine
2025;25(12):1921-1930
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish a stable rat model of sequelae of pelvic inflammatory disease(SPID)with clinical characteristics,and to provide a reliable experimental model for the study of the pharmcological effect and mechanism of SPID.Methods:Twenty-four 7-week-old SD rats were divided into sham operation group,model-A(108 cfu/mL mixed bacterial solution,0.2 mL),model-B(109 cfu/mL mixed bacterial solution 0.2 mL),and model-C(108 cfu/mL E.coli 0.2 mL).The weight of the rat's uterine was weighed and the uterine index was calculated.The automatic hematology analyzer was used to detect the blood routine;hematoxylin-eosin staining(HE)and masson staining were used to detect uterine pathlogical changes in rats.Enzyme-linked immunosorbent assay(ELISA)was used to detect interleukin-1β(IL-1β),interleukin-6(IL-6)and tumor necrosis factor-α(TNF-α)in rat uterine tissue homogenates.Western blot was used to detect the expression of proteins related to NF-κB signaling pathway.Results:Compared with the sham operation group,the uterine index of model-A,model-B,and model-C were significantly increased(P＜0.05,P＜0.01).The levels of WBC and NE in the model-A increased significantly(P＜0.01).The level of LY in model-B decreased significantly(P＜0.01).The levels of IL-1β,TNF-α in model-A,model-B,and model-C were significantly increased(P＜0.01).The levels of IL-6 in model-A and model-B were significantly increased(P＜0.05,P＜0.01).The collagen volume fraction of model-A and model-B were significantly increased(P＜0.01).Mechanism study indicates that the expression levels of p-IKKβ/IKKβ,p-IκBα/IκBα and p-p65/p65 in model-A were significantly increased(P＜0.01),and the expression levels of IκBα/β-actin were significantly decreased(P＜0.01).The expression level of p-IKKβ/IKKβ in model-B was significantly increased(P＜0.01).Conclusions:A stable rat model of SPID that conforms to clinical characteristics can be successfully constructed by combining 0.2 mL of mixed bacterial solution with a concentration of 108 cfu/mL and mechanical injury.This modeling method intervened in the expression of the NF-κB inflammatory signaling pathway.
